Caridoid escape reaction - AbsoluteAstronomy.com

The Caridoid Escape Reaction, also known as lobstering or tail-flipping, refers to an innate escape mechanism in marine and freshwater crustaceans such as lobsters, krill

Krill

Krill is the common name given to the order Euphausiacea of shrimp-like marine crustaceans. Also known as euphausiids, these small invertebrates are found in all oceans of the world...

, shrimp

Shrimp

Shrimp are swimming, decapod crustaceans classified in the infraorder Caridea, found widely around the world in both fresh and salt water. Adult shrimp are filter feeding benthic animals living close to the bottom. They can live in schools and can swim rapidly backwards. Shrimp are an important...

and crayfish

Crayfish

Crayfish, crawfish, or crawdads – members of the superfamilies Astacoidea and Parastacoidea – are freshwater crustaceans resembling small lobsters, to which they are related...

.

The reaction, most extensively researched in crayfish, allows crustaceans to escape predators through rapid abdominal flexions that produce powerful swimming strokes — thrusting the crustacean through the water and away from danger. The type of response depends on the part of the crustacean stimulated, but this stereotyped behavior is complex and is regulated both spatially and temporally through the interactions of several neurons.

Discovery of the First Command Neuron Mediated Behavior

In 1946, C.A.G. Wiersma first described the tail-flip escape in the crayfish Procambarus clarkii
Procambarus clarkii
Procambarus clarkii is a freshwater crayfish species, native to the Southeastern United States, but found also on other continents, where it is often an invasive pest. It is known variously as the red swamp crawfish, red swamp crayfish, Louisiana crawfish, Louisiana crayfish or mudbug.-Range and...

and noted that the giant interneurons present in the tail were responsible for the reaction. The aforementioned neuronal fibres consist of a pair of lateral giant interneurons and a pair of medial giant interneurons, and Wiersma found that stimulating just one Lateral Giant Interneuron (LG or LGI) or one Medial Giant Interneuron
Medial giant interneuron
The medial giant interneuron is an interneuron in the abdominal nerve cord of crayfish. It is part of the system that controls the caridoid escape reaction of crayfish, clawed lobsters, and other decapod crustaceans...

(MG or MGI) would result in the execution of a tail flip. Wiersma and K. Ikeda then proposed the term “Command Neuron” in their 1964 publication, and applied it to the giant interneurons ability to command the expression of the escape response. This was the first description of a command neuron

Command neuron

A command neuron is a single neuron whose stimulation results in the evocation of an endogenous, specific, naturally occurring behavior pattern . Command neurons act as neural decision making cells; push buttons that can trigger a complete, coordinated behavioral act and are often the sole...

mediated behavior and it indicated that the depolarization

Depolarization

In biology, depolarization is a change in a cell's membrane potential, making it more positive, or less negative. In neurons and some other cells, a large enough depolarization may result in an action potential...

of a neuron could precipitate complex innate behaviors in some organisms.

This concept was further fleshed out with more specific and stringent conditions in 1972 when Kupfermann and Weiss published The Command Neuron Concept. The paper stated that command neurons were neurons (or small sets of neurons) carrying the entire command signal for a natural behavioral response. According to the authors, command neurons were both necessary and sufficient in the production of a behavioral response. The concept of command neuron mediated behaviors was both ground breaking and controversial, since determining command neuron mediated behaviors was a problematic process due to difficulties in understanding and interpreting anatomical and behavioral data.

Research with Crayfish

Behavioral neurobiologists in the field of neuroethology

Neuroethology

Neuroethology is the evolutionary and comparative approach to the study of animal behavior and its underlying mechanistic control by the nervous system...

have researched this behavior extensively for over fifty years in the crayfish species Procambarus clarkii
Procambarus clarkii
Procambarus clarkii is a freshwater crayfish species, native to the Southeastern United States, but found also on other continents, where it is often an invasive pest. It is known variously as the red swamp crawfish, red swamp crayfish, Louisiana crawfish, Louisiana crayfish or mudbug.-Range and...

. Based on studies of P. clarkii it was discovered that the tail- flip mechanism is characterized by a decisive, all-or-nothing quality that inhibits all unnecessary behaviors while generating a fixed action pattern

Fixed action pattern

In ethology, a fixed action pattern , or modal action pattern, is an instinctive behavioral sequence that is indivisible and runs to completion...

for escape swimming. The type of escape response

Escape response

Escape response, escape reaction, or escape behaviour is a possible reaction in response to stimuli indicative of danger, in particular, it initiates an escape motion of an animal...

depends on the region of the crayfish that is stimulated but all forms require abdominal contractions. When a strong, unpleasant tactile stimulus is presented, such as a burst of water or the prod of a probe, a stereotyped behavior occurs in which the muscular tail and wide tail fan region of the telson

Telson

The telson is the last division of the body of a crustacean. It is not considered a true segment because it does not arise in the embryo from teloblast areas as do real segments. It never carries any appendages, but a forked "tail" called the caudal furca is often present. Together with the...

are used like a paddle to propel the crustacean away from harm using a powerful abdominal flexions. The entire process occurs in a fraction of a second as movements are generated within two hundredths of a second (20 milliseconds) from the original trigger stimulus and the period of latency after a flexion is a hundredth of a second (10 milliseconds). Finally, the caridoid escape reflex requires that neurons be able to complete the arduous task of synchronizing the flexion of several abdominal segments. The speed, coordination, and decisiveness of the process seem to be the main attributes to its success.

Anatomy involved

Like other decapod

Decapoda

The decapods or Decapoda are an order of crustaceans within the class Malacostraca, including many familiar groups, such as crayfish, crabs, lobsters, prawns and shrimp. Most decapods are scavengers. It is estimated that the order contains nearly 15,000 species in around 2,700 genera, with...

crustaceans, the crayfish possesses a hard, segmented exoskeleton

Exoskeleton

An exoskeleton is the external skeleton that supports and protects an animal's body, in contrast to the internal skeleton of, for example, a human. In popular usage, some of the larger kinds of exoskeletons are known as "shells". Examples of exoskeleton animals include insects such as grasshoppers...

that reflects muscular and neural segmentation. The anterior portion of the crayfish is the cephalothorax region. The region rostral to the cephalic grove, which separates the head and thorax region, is characterized by the presence of eyes, antennae and claws while the region caudal contains four pairs of walking legs. This is the crayfish’s primary mode of locomotion. The abdominal section of the crayfish is divided into seven segments. These segments are flexibly interconnected, forming the tail. Normally, the tail is held in an extended position to aid in maneuvering and balancing. The first five segments are similar and the two terminal segments are modified into a tail fan, a region with high surface area that acts as the blade of a paddle in the escape response. This region contains the telson

Telson

. The abdominal segments contain swimmerets , which aid in swimming.

The anterior five segments of the crayfish house the massive flexor and extensor muscle

Muscle

Muscle is a contractile tissue of animals and is derived from the mesodermal layer of embryonic germ cells. Muscle cells contain contractile filaments that move past each other and change the size of the cell. They are classified as skeletal, cardiac, or smooth muscles. Their function is to...

s. Six abdominal ganglia run down the entire length of the abdomen and communicate with one another through projections. The first five abdominal segments each have their own ganglion, that contains three roots with outward projections. The first has mixed sensory and motor nerves innervating swimmerets while the second has sensory and motor neuron

Motor neuron

In vertebrates, the term motor neuron classically applies to neurons located in the central nervous system that project their axons outside the CNS and directly or indirectly control muscles...

s that innervate the extensor muscles, while the third root contains only motor neuron projections that extend into the flexor muscles. The last segment contains the fusion of two ganglia. The ganglia here also receive sensory input from the sensitive hairs on the tail fan.

Each ganglia contains the body of one Motor Giant Neurons (MoG) , powerful and large bodied motor neurons whose projections innervate the five Fast Flexor (FF) muscles found in a segment and interact with them through chemical synapse

Chemical synapse

Chemical synapses are specialized junctions through which neurons signal to each other and to non-neuronal cells such as those in muscles or glands. Chemical synapses allow neurons to form circuits within the central nervous system. They are crucial to the biological computations that underlie...

s. The ganglia also contain two sets of giant axon

Axon

An axon is a long, slender projection of a nerve cell, or neuron, that conducts electrical impulses away from the neuron's cell body or soma....

s known as the lateral giant neurons and the medial giant neurons. These interneurons play important roles in escape swimming. Their large diameter allows for rapid conduction since there is less current leakage. Their projections extend through the third root in each ganglion, and Furshpan and Potter found that they synapses they subsequently made with the MoG passed depolarizing currents in a direct and unidirectional manner. These electrical synapses account for the speed of the escape mechanism and display some features of chemical synapse

Chemical synapse

s such as LTP and LTD. Variations in escape response characteristic depend on the location where the crayfish body is prodded or attacked and also depend on which of the giant neurons is stimulated.

Medial Giant Mediated Escape

The medial giants innervate the entire ventral nerve chord. Their cell bodies and dendrite

Dendrite

Dendrites are the branched projections of a neuron that act to conduct the electrochemical stimulation received from other neural cells to the cell body, or soma, of the neuron from which the dendrites project...

s begin in the brain and collect sensory information presented by visual and tactile stimuli

Stimulus (physiology)

In physiology, a stimulus is a detectable change in the internal or external environment. The ability of an organism or organ to respond to external stimuli is called sensitivity....

. These fibers terminate in the last abdominal segment. The response is triggered by abrupt tactile stimuli to the head or alarming visual stimuli. This results in the firing of all Motor Giant (MoG) neurons and the flexion of all the phasic Fast Flexor (FF) muscles in the abdomen, which rapidly curls the tail fan and last three segments underneath the crayfish. This produces a thrust that directs water towards the offending stimulus while propelling the crayfish directly backward. This behavior is then followed by the extension of the tail, which prepares it for swimming or the execution of another tail-flip. The extension and swimming phases will be discussed in the next escape variant.

Unfortunately, this variant of the tail-flip escape has not been extensively studied. Further studies should focus on this escape variant, paying special attention to exactly how visual information is processed and then converted into neuronal signals that produce a tail flip response.

Non-giant escape

Less abrupt or gradually intensifying stimuli evoke locomotion behavior similar to that seen in giant interneuron mediated behaviors. However, the movements are less stereotyped and do not appear to occur in an all or nothing manner. They are so named because they lack the involvement of the giant interneurons, most likely because they do not produce depolarizations in the sensory neurons that are above the thresholds required to initiate these behaviors. Instead they involve one or a few of the smaller neurons innervating the tail. The latency periods for these escape mechanisms are longer, ranging from 80 to 500 ms. However, this slower swimming behavior allows for flexibility, since the crayfish can use visual stimuli and steer itself in a selected direction.

Non-giant escape often occurs during situations where Lateral or Medial Giant mediated escape may not be beneficial or during times where those behaviors are suppressed. This allows the crayfish to attack offenders, escape during feeding, or wriggle free when it has been restrained by the carapace.

Non-giant escape circuitry was found to be activated more during frontal attacks, but was rarely involved in the initial escape during a rear attack. Non-giant escape is often used after an initial giant interneuron mediated tail flip. Compared to MG escape, the potentials produced in the MoG neurons by non-giant circuitry have lower amplitudes and longer durations while the recordings in the FF muscles are more erratic and have smaller amplitudes since they receive smaller EPSPs from the MoGs. So while it is hard to observe differences in MG and non-escapes, this property can be used to distinguish the two.

Lateral Giant Mediated Escape

The Lateral Giant (LG) mediated escape mechanism is the most extensively analyzed form of the tail flip. The LG is not actually one neuron, but rather a group of closely associated neurons arranged end to end and connected by electrical synapses (also called septate synapses). As a result, the LGI functions as one giant, continuous neuron, such as the MG. The LGI’s large diameter and the efficiency and speed of electrical synapses, make the LGI mediated escape especially rapid and effective. The LGI only innervates the first three rostral segments of the tail and is activated within 10 ms from when a mechanical stimulus is presented to the abdomen. When flexion occurs, only the first three segments flex and the tail fan is not directed under the body and forward but rather straight down. The unflexed segments increase the length of the paddle. The result is the rear end of the animal is directed upward and forward causing the animal to tumble or somersault forward. The tail is then rapidly extended, and this is usually followed by directed swimming.

The LGI is one of the few examples of a true command neuron. It passes the necessity and sufficiency principle put forward by Kupfermann and Weiss. Wiersma’s initial experiments showed that direct stimulation of the LGI was a sufficient release for the tail flip. In 1981, it was Olson and Krasne who showed that the LGI fulfilled the condition of necessity, because when spikes were inhibited in the LGI through introducing a hyperpolarizing current, no motor activity was produced in response to a stimulus that would have originally elicited the tail flip.

The LGI contains the following three phases which occur over the span of about 100ms:

1 — Flexion production

The LGI receives its sensory input from the hair-like projections found on the edge of the tail fan. The sensory information is sent from bipolar receptors connected to the hair that are directionally sensitive. The path of the signal varies from here depending on the strength of the input. In the beta pathway, the signal can then pass a chemical synapse, the information is sent to a Sensory Interneuron (SI) of either type A (which fires phasically in response to input) or type C, (which fire tonically). From there, the signal is sent across an electrical synapse to the LGI. The LGI can also receive the sensory input directly through an electrical synapse by way of the alpha pathway. This bypasses the SIs.The LGI can pass the signal to the MoG’s by two paths one of which passes the signal to the Motor Giant through direct electrical synapses. The MoG then passes the signal to the FF muscles. It can also use a second route across a fast electrical synapse to a premotor interneuron called the Segmental Giant (SG), two of which are located in each segment. The SGs then relays the signal to fast flexor motor neurons. The end result is a powerful and rapid flexion.

The convergence many inputs on the LGI and divergence of few signals show how the LGI functions as a decision making coincidence detector. It takes the information from both the alpha and the beta pathways and if the timing of the spikes is synchronous, the behavior is produced. If the timing is asynchronous, the later input is blocked by reducing the driving force of the signal and increasing the threshold voltage. The early synapse also causes current to leak through the active synapse, resulting in weak EPSPs that are ultimately unable to generate the tail flip.

A number of processes are inhibited once the initial flexion has begun. Further LGI spiking, influx of sensory information from the tail, MoG spiking and FF contraction are all inhibited so that a flexion and extension cycle can be completed before another flexion begins. Long IPSPs are generated to prevent these events. The extensor muscles are inhibited to prevent competition between the extensor and flexors. Rapid IPSPs presented at the muscle receptor organ (MRO) prevent the stretch receptor from initiating extension while they are also presented at the fast extensor motor neurons and the fast extensor muscles. The circuits responsible for slow flexion and extension are also inhibited.

2 — Reextension

When the LGI mediated inhibition subsides, the reextension process begins. This process is mediated by the MRO and tail fan hair receptors, which were inhibited during the flexion portion of the escape behavior. The MRO is responsible for the initiation of extension and the inhibition of flexion through a reflex like mechanism.The muscle fibers of the MRO are located on the dorsal side of the abdomen, each spanning the joint of two segments. They are innervated by proprioreceptors that detect the stretch in extensor muscles when the flexors are contracted. They then fire phasically or tonically. It then excites the motor neurons in the fast extensor muscles while directly exciting an inhibitory neuron that prevents contractions in the FF muscles. The hair cell

Hair cell

Hair cells are the sensory receptors of both the auditory system and the vestibular system in all vertebrates. In mammals, the auditory hair cells are located within the organ of Corti on a thin basilar membrane in the cochlea of the inner ear...

s detect the resulting movement caused by the tail flip when activated, they would fire and excite the fast extensor motor neurons.

3 — Swimming

Non-giant mediated responses are initiated after the tail flip , creating cycles of flexion followed by extension. This non-giant system is activated parallel to the LGI circuit when the hair cells receive input. However, this behavior has a longer delay that allows the onset of swimming to occur after the completion of the tail flip. The non-giant swimming occurs independently of the LGI response since direct stimulation of the LGI with the electrodes results in a tail flip but not the subsequent non-giant swimming. This swimming response seems to be a fixed action pattern mediated by a central pattern generator since it does not require sensory feedback for physical and temporal maintenance.

Modulation and plasticity of the response

The production of a tail flip is not always beneficial. Evolution has allowed the crayfish to be more flexible by presenting several control systems that will prevent the tail flip in situations where it will most likely be unnecessary or ineffective.

Eating

Crayish often find themselves in a conflicting situation where they are performing the highly motivated behavior of feeding when they suddenly receive a tail flip stimulus. Often, the crayfish will not perform a tail flip in this situation. This is because when a crayfish is actively feeding, the LGI itself is modulated by the behavior. Feeding increases the threshold for the tail flip, and even when the tail is tapped, no spikes are initiated. As a result, it takes an especially strong stimulus to elicit the tail flip. This prevents the crayfish from going hungry unless it is absolutely necessary.

Carapace restraint

When a crayfish is held by its carapace either in the water or in the air, it does not respond with a tail flip when it receives sensory stimuli that would normally elicit the response. This inhibitory behavior does not seem to mediated by the abdomen. When the thorax-abdomen junction is severed, the inhibitory effect is lost and the LGI in severed abdomen generates strong spikes since its threshold had decreased substantially. This is an indication that the behavior is mediated in the brain or the thorax of the crayfish. Most non-giant and MGI tail flips are also suppressed restraint, so only a few non-giant systems are active to allow the crayfish to carefully wriggle free. The setup of this system seems to be designed to prevent the unnecessary use of all or nothing tail flips or ineffective tail flips when the situation requires more careful maneuvering.

The tail flip normally induces inhibition in an absolute fashion, such that it takes precedence over all the other tasks the segments are performing. However during these situations, the effectiveness of the tail flip is reduced, so the inhibitory processes need to occur in a relative manner and a tail flip should occur only if absolutely necessary. During absolute inhibition is directed by more proximal synapses controlling the spike initiating zones of the LGI.
It is most likely that during restraint or feeding, the inhibitory process is mediated by inhibitory synaptic input on distal dendrites of the LGI . As a result, these signals would have to compete with other inputs for the control of the LGI.

Learning

Repeated tapping of the abdomen leads to habituation of the tail flip mechanism. However, self–habituation is prevented by command neuron–derived inhibition because when a tail flip is begun, the mechanisms that induce habituation are repressed. The habituation occurs at the level of the A type and C type interneurons, which experience synaptic depression. The habituation process is also mediated further up the circuit through the buildup of tonic inhibition, brought on by the repeated stimulation.

Social status

The feedback from social situations affect a crayfish’s ability to perform a tail flip. Serotonin levels are affected by social status. High levels are associated with aggressive behavior and a reduction in the frequency of tail flips performed. This is because the excitability of the LGI is decreased. Aggressive dominant males have a moderate reduction in tail flips while the subordinates have a much lower occurrence of tail flips. This presents a paradox since this means that subordinates are more likely to get killed. However, it was found that subordinates are more likely to use non giant mediated escape, indicating that the reduction in tail flips and enhancement of non giant escape is adaptive.

Changes in social status should correlate with changes in serotonin levels, resulting in changes in the escape strategies used by the crayfish. However this is only true when submissive crayfish become dominant, and not the reverse. The neuromodulatory processes of facilitation and inhibition seem to be mediated by different cell receptors. Differences in the effects of serotonin on the behavior of the crayfish seem to be the result of differences in the populations of these receptors. It is unknown how these modulation processes convey the information to the LG, and how the behavioral changes are precipitated.

Evolution of the tail flip escape mechanism

It has been hypothesized that the tail flip is derived from an ancient limb protraction driven (as opposed to tail flexion-driven) mechanism. This is because the SG’s appear to be modified limb motor neurons whose peripheral axons affect the legs and swimmerets, but end blindly without any known function. It is known that another effect of Giant Fiber excitation is limb promotion which suggests that the premotor limb interneurons may be ancestors of the Giant Fibers.

It has been speculated that the ancestral escape mechanism was most likely a backwards jump due to the simultaneous protraction of the legs driven by the ancestors of the Giant Fibers. This behavior was probably similar to the escape system found in a mantis shrimp

Mantis shrimp

Mantis shrimp or stomatopods are marine crustaceans, the members of the order Stomatopoda. They are neither shrimp nor mantids, but receive their name purely from the physical resemblance to both the terrestrial praying mantis and the shrimp. They may reach in length, although exceptional cases of...

called Squilla that diverged from the crayfish lineage very early on. It is likely that this mechanism was replaced by the tail flip since the wide surface area of the tail made this behavior more selectively advantageous. This most likely occurred when the ancestral flexor motor neurons in each segment formed connections with one of these limb motor neurons. The Squilla mechanism seems be similar to this ancestral state because a large diameter axon in the dorsal nerve chord facilitates limb promoter motor neurons. This seems to match the ancestral condition, but it is not known for sure whether the circuitry is homologous.

External links

The Lobster Conservatory includes information on the biology and conservation of lobsters. The majority can be applied to crayfish due to common ancestry and homology.
- Neural and tail anatomy provides an idea of the organization of the segmental ganglia in the tail of the crayfish. The second diagram on the page is a transverse section through the tail that highlights the positions of the LGI, MGI and non-giant neurons. At the bottom of the page it also has diagrams of the tail flips caused by stimulation of the LGI (on the left half of the diagram) and the MGI (on the right half of the diagram). This accurate diagram appears to be similar to that found in Wine and Krasne’s 1972 publication.
- Lobster Body Plan presents information on decapod
  Decapoda
  The decapods or Decapoda are an order of crustaceans within the class Malacostraca, including many familiar groups, such as crayfish, crabs, lobsters, prawns and shrimp. Most decapods are scavengers. It is estimated that the order contains nearly 15,000 species in around 2,700 genera, with...
  
  anatomy.
Cornell University's Neuroethology Course course page

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