Kinetic capillary electrophoresis
Encyclopedia
Kinetic capillary electrophoresis or KCE is capillary electrophoresis
of molecule
s that interact during electrophoresis. KCE was introduced and developed by Professor Sergey Krylov and his research group at York University
, Toronto, Canada. It serves as a conceptual platform for development of homogeneous chemical affinity
methods for studies of molecular interactions (measurements of binding and rate constants) and affinity purification (purification of known molecules and search for unknown molecules). Different KCE methods are designed by varying initial and boundary conditions – the way interacting molecules enter and exit the capillary. Several KCE methods were described: non-equilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM), sweeping capillary electrophoresis (SweepCE), plug-plug KCE (ppKCE).. More detailed description and several applications of KCE methods (measuring equilibrium and rate constants of molecular interactions, quantitative affinity analysis of proteins, thermochemistry of protein–ligand interactions, selection of aptamer
s, determination of temperature inside a capillary) can be found in a PDF presentation: KCE ia a conceptual platform for kinetic homogeneous affinity methods.
Capillary electrophoresis
Capillary electrophoresis , also known as capillary zone electrophoresis , can be used to separate ionic species by their charge and frictional forces and hydrodynamic radius. In traditional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an...
of molecule
Molecule
A molecule is an electrically neutral group of at least two atoms held together by covalent chemical bonds. Molecules are distinguished from ions by their electrical charge...
s that interact during electrophoresis. KCE was introduced and developed by Professor Sergey Krylov and his research group at York University
York University
York University is a public research university in Toronto, Ontario, Canada. It is Canada's third-largest university, Ontario's second-largest graduate school, and Canada's leading interdisciplinary university....
, Toronto, Canada. It serves as a conceptual platform for development of homogeneous chemical affinity
Chemical affinity
In chemical physics and physical chemistry, chemical affinity is the electronic property by which dissimilar chemical species are capable of forming chemical compounds...
methods for studies of molecular interactions (measurements of binding and rate constants) and affinity purification (purification of known molecules and search for unknown molecules). Different KCE methods are designed by varying initial and boundary conditions – the way interacting molecules enter and exit the capillary. Several KCE methods were described: non-equilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM), sweeping capillary electrophoresis (SweepCE), plug-plug KCE (ppKCE).. More detailed description and several applications of KCE methods (measuring equilibrium and rate constants of molecular interactions, quantitative affinity analysis of proteins, thermochemistry of protein–ligand interactions, selection of aptamer
Aptamer
Aptamers are oligonucleic acid or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist in riboswitches. Aptamers can be used for both basic research and clinical purposes as...
s, determination of temperature inside a capillary) can be found in a PDF presentation: KCE ia a conceptual platform for kinetic homogeneous affinity methods.