Cannula transfer
Cannula transfer or cannulation is a subset of air-free technique
Air-free technique
Air-free techniques refer to a range of manipulations in the chemistry laboratory for the handling of compounds that are air-sensitive. These techniques prevent the compounds from reacting with components of air, usually water and oxygen; less commonly carbon dioxide and nitrogen...

s used with a Schlenk line
Schlenk line
225px|thumb|Vacuum gas manifold set up: 1 inert gas in, 2 inert gas out , 3 vacuum 4 reaction line, 5 Teflon tap to gas, 6 Teflon tap to vacuum 225px|thumb| Vacuum gas manifold set up: 1 inert gas in, 2 inert gas out , 3 vacuum , 4 reaction line, 5 double oblique stopcock...

, in transferring liquid or solution samples between reaction vessels via cannula
A cannula or canula is a tube that can be inserted into the body, often for the delivery or removal of fluid or for the gathering of data...

e, avoiding atmospheric contamination. While the syringes are not the same as cannulae, the techniques remain relevant.

There are two methods of transfer: vacuum, and pressure. Both utilize differences in pressures between two vessels to push the fluid through. Often, the main difficulty encountered is slow transfer due to the high viscosity
Viscosity is a measure of the resistance of a fluid which is being deformed by either shear or tensile stress. In everyday terms , viscosity is "thickness" or "internal friction". Thus, water is "thin", having a lower viscosity, while honey is "thick", having a higher viscosity...

 of the fluid.


Septa (singular: septum
In anatomy, a septum is a wall, dividing a cavity or structure into smaller ones.-In human anatomy:...

) are rubber stoppers which seal flasks or bottles. They give an air-tight seal, preventing the ingress of the atmosphere, but are able to be pierced by sharp needles or cannulae.


Cannulae are hollow flexible tubes of varying bore, usually 16-22 gauge
Wire gauge
Wire gauge is a measurement of how large a wire is, either in diameter or cross sectional area. This determines the amount of electric current a wire can safely carry, as well as its electrical resistance and weight per unit of length...

 thick. They are commonly made of stainless steel
Stainless steel
In metallurgy, stainless steel, also known as inox steel or inox from French "inoxydable", is defined as a steel alloy with a minimum of 10.5 or 11% chromium content by mass....

 or PTFE for chemical resistance. Stainless steel cannulae are usually 2-3 feet long, due to their relative inflexibility, while PTFE cannulae can be much shorter. The ends are usually sharp and non-coring, allowing them to easily pierce a rubber septum
In anatomy, a septum is a wall, dividing a cavity or structure into smaller ones.-In human anatomy:...

, without being clogged by rubber particles. Flat ends may be chosen on occasion, because they tend to provide more complete transfer of fluids.

Stainless steel cannulae tend to collapse when cut with wire cutters. They are best cut using pipecutter
A pipecutter is a type of tool used by plumbers to cut pipe. Besides producing a clean cut, the tool is often a faster, cleaner, and more convenient way of cutting pipe than using a hacksaw, although this depends on the metal of the pipe....

s of appropriate size. Other workers recommend deeply scoring the cannula with a triangular file, then sharply snapping the weakened section.

Needles and syringes

Wide-bore needles of similar gauge are often used. Unlike hypodermic-type needles
Hypodermic needle
A hypodermic needle is a hollow needle commonly used with a syringe to inject substances into the body or extract fluids from it...

 sometimes used in the chemistry laboratory, these needles tend to be reused due to cost. Long needles may be flexible enough to be bent in U-shapes; shorter needles often are not.

Polypropylene , also known as polypropene, is a thermoplastic polymer used in a wide variety of applications including packaging, textiles , stationery, plastic parts and reusable containers of various types, laboratory equipment, loudspeakers, automotive components, and polymer banknotes...

 syringes used for medical applications are least expensive. While the material is relatively solvent-resistant, though they are designed primarily for aqueous solutions, some degradation or leaching by the contents may occur. In particular, the black rubber seal may swell and cause the plunger to seize.

All-glass gas-tight syringes have better solvent resistance, though they tend to leak more than plastic syringes. Greases used on the barrel may leach into the contents. Glass syringes with a teflon seal at the plunger are available as well, but they are more expensive. They tend to be used for microsyringes (usually containing less than 100 µL). Luer fittings are preferred, as needles are locked in even under higher pressure, e.g. when transferring viscous liquids.

Cleaning and storage

Cannulae and needles should be quickly flushed out with an appropriate solvent to prevent undetectable corrosion damage to the stainless steel. Since they are usually used for air-sensitive work, they are commonly kept in a hot oven, to reduce the adsorption of water molecules. Before use, they are usually subjected to three vacuum-refill cycles to remove any traces of air.

Vacuum based

The two ends of the cannula are inserted through the septa covering donating and receiving flasks. The cannula extends below the surface of the fluid to be transferred. A vacuum is applied to the receiving flask, and the low pressure relative to the donating flask causes the fluid to flow through the cannula.

The main drawback of vacuum transfer is that if there are any leaks, air will be drawn into the system and spoil the air-free environment. Loss of the fluid by evaporation is another problem, though less so where the fluid is a neat liquid, than a solution of known concentration.

Positive pressure

The two ends of the cannula are connected similarly. The receiving flask is connected to its own gas bubbler, while the donating flask is connected to a source of inert gas. By increasing the inert gas pressure, the pressure within the donating flask is raised higher than the receiving flask, and the fluid is forced through the cannula.

The main drawback of pressure transfer, is that transfer can be slow due to the small pressure difference between donating and receiving flasks. Inert gas lines are usually vented out of a gas bubbler placed in-line to prevent overpressure. The vents need to be isolated by capping the bubbler outlet, or stopping the egress of inert gas with a stopcock or pinch clamp, to ensure sufficient pressure to complete the transfer. The use of a mercury bubbler instead of one filled with oil used to be popular, but is out of favor due to the difficulty in dealing with mercury spills.


By carefully filling the cannula fully with either above techniques, then allowing the pressures within the vessels to equalize, a syphon may be set up. This arrangement allows the slow addition of a fluid to a reaction vessel; the rate of addition may be controlled by adjusting the relative height of the donor vessel.

Syringe transfer

Fluids may also be transferred via syringes; at all times a slight over-pressure of inert gas is maintained to prevent the ingress of moisture or air, as well as providing refill volume. This method is also applicable for transferring fluids from bottles similar to the Aldrich Sure/Seal-type.

Prior to withdrawing the needle for subsequent transfer, the needle bore is usually filled with inert gas to serve as a buffer from the atmosphere.

Handling pyrophoric material

While handling pyrophoric material (e.g. tert-butyllithium
tert-Butyllithium is a chemical compound with the formula 3CLi. As an organometallic compound, it has applications in organic synthesis since it is a sufficiently strong base to deprotonate many carbon acids, including benzene...

 and trimethylaluminum), traces of the compound at the tip of the needle or cannula may catch fire, and cause a clog. Some workers prefer to contain the tip of the needle or cannula in a short glass tube flushed with an inert gas, and sealed via two septa.

Instead of exposing the needle tip to the air, it is withdrawn into the inerted tube. Where desired, it may be inserted into a flask via two septa (one on the tube, one on the flask). Used this way, needle tip fires are eliminated, reducing the obvious hazards. Also, there is a reduced tendency for the needle tip to clog due to the reaction of traces of the reagent with air to give salts.


Filtration is most easily accomplished using a syringe filter. PTFE filters tend to be most chemically resistant; nylon filters are less so.

Using a cannula, a filter stick Air-free technique#Gallery may be used. A filter stick is a short length of glass tubing sealed on one end with a septum, and sealed on the other with filter paper, or a sintered glass frit.

For larger volumes, it may be preferable to connect the donor and receiving flasks via ground glass joints to a sintered glass filter tube.


Air-sensitive cannulas:

1: Pressure in (gas in) 2: Pressure out (oil bubbler orange) 3: Higher flask with transfer liquid (yellow) to transfer 4: Lower receiving flask/transferred liquid (yellow)

5: Liquid transfer cannula 6: Septum (orange) on transfer flask 7: Septum (orange) on receiving flask 8: Pressure-control regulator/stopcock

9: Tubing/ gas-line (not shown for clarity, arrows show connectivity) 10: Gas cannula 11: 2-way syringe stopcock 12: Gas-tight syringe

13: Gas/pressure removed from flask 3 14: Gas/pressure added to flask 4

O = Open stopcock; X = Closed stopcock; black-arrow = Gas flow direction, orange arrow = Liquid flow direction
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