CLED agar
Encyclopedia
CLED agar is a valuable non-inhibitory growth medium
Growth medium
A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens.There are different types of media for growing different types of cells....

 used in the isolation and differentiation of urinary
Urine
Urine is a typically sterile liquid by-product of the body that is secreted by the kidneys through a process called urination and excreted through the urethra. Cellular metabolism generates numerous by-products, many rich in nitrogen, that require elimination from the bloodstream...

 organisms. Being electrolyte deficient, it prevents the swarming of Proteus
Proteus (bacterium)
Proteus is a genus of Gram-negative Proteobacteria.-Clinical significance:Three species—P. vulgaris, P. mirabilis, and P. penneri—are opportunistic human pathogens. Proteus includes pathogens responsible for many human urinary tract infections. P. mirabilis causes wound and urinary...

species. Cystine promotes the formation of cystine-dependent dwarf colonies. Lactose fermenters produce yellow colonies
Colony (biology)
In biology, a colony reference to several individual organisms of the same species living closely together, usually for mutual benefit, such as stronger defense or the ability to attack bigger prey. Some insects live only in colonies...

 on CLED agar; non-lactose fermenters appear blue. It has a pH
PH
In chemistry, pH is a measure of the acidity or basicity of an aqueous solution. Pure water is said to be neutral, with a pH close to 7.0 at . Solutions with a pH less than 7 are said to be acidic and solutions with a pH greater than 7 are basic or alkaline...

 of approximately 7.3. CLED agar contains:
Peptone
Peptide
Peptides are short polymers of amino acid monomers linked by peptide bonds. They are distinguished from proteins on the basis of size, typically containing less than 50 monomer units. The shortest peptides are dipeptides, consisting of two amino acids joined by a single peptide bond...

4g/l
'Lab Lemco' powder
'Lab Lemco' powder
Lab Lemco' Powder is a refined meat extract that has been in production since 1865 of very light colour. This product is used in a wide range of bacteriological growth media. It has excellent growth-promoting qualities, and is much easier to handle than most other meat extracts....

3g/l
Tryptone
Tryptone
Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.Tryptone is commonly used in microbiology to produce Lysogeny broth for the growth of E. coli and other microorganisms...

4g/l
Lactose
Lactose
Lactose is a disaccharide sugar that is found most notably in milk and is formed from galactose and glucose. Lactose makes up around 2~8% of milk , although the amount varies among species and individuals. It is extracted from sweet or sour whey. The name comes from or , the Latin word for milk,...

10g/l
L-Cystine
Cystine
Cystine is a dimeric amino acid formed by the oxidation of two cysteine residues that covalently link to make a disulfide bond. This organosulfur compound has the formula 2. It is a white solid, and melts at 247-249 °C...

128 mg/l
Bromothymol blue
Bromothymol blue
Bromothymol blue is a chemical indicator for weak acids and bases. The chemical is also used for observing photosynthetic activities or respiratory indicators .Bromothymol blue acts as a weak acid in solution...

20 mg/l
Agar
Agar
Agar or agar-agar is a gelatinous substance derived from a polysaccharide that accumulates in the cell walls of agarophyte red algae. Throughout history into modern times, agar has been chiefly used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture medium...

No. 1
15g/l
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