Bovine serum albumin
Encyclopedia
Bovine serum albumin is a serum albumin
protein derived from cows. It is often used as a protein concentration standard.
The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology
that made use of differential solubility characteristics of plasma proteins. By manipulating solvent concentrations, pH, salt
levels, and temperature
, Cohn was able to pull out successive "fractions" of blood plasma
. The process was first commercialized with human albumin for medical use and later adopted for production of BSA.
is cut off from the precursor protein upon secretion, hence the initial protein product contains 589 amino acid residues. An additional 4 amino acids is cleaved to yield the mature BSA protein that contains 585 amino acids.
Physical properties of BSA:
s (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry
. It is also used as a nutrient in cell and microbial culture. In restriction digest
s, BSA is used to stabilize some enzymes during digestion
of DNA
and to prevent adhesion of the enzyme to reaction tubes and other vessels
. This protein
does not affect other enzyme
s that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA (see Bradford protein assay
). BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
Serum albumin
Serum albumin, often referred to simply as albumin is a protein that in humans is encoded by the ALB gene.Serum albumin is the most abundant plasma protein in mammals. Albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular...
protein derived from cows. It is often used as a protein concentration standard.
The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology
Cohn process
The Cohn process is a series of purification steps with the purpose of extracting albumin from blood plasma. The process is based on the differential solubility of albumin and other plasma proteins based on pH, ethanol concentration, temperature, ionic strength, and protein concentration. Albumin...
that made use of differential solubility characteristics of plasma proteins. By manipulating solvent concentrations, pH, salt
Salt
In chemistry, salts are ionic compounds that result from the neutralization reaction of an acid and a base. They are composed of cations and anions so that the product is electrically neutral...
levels, and temperature
Temperature
Temperature is a physical property of matter that quantitatively expresses the common notions of hot and cold. Objects of low temperature are cold, while various degrees of higher temperatures are referred to as warm or hot...
, Cohn was able to pull out successive "fractions" of blood plasma
Blood plasma
Blood plasma is the straw-colored liquid component of blood in which the blood cells in whole blood are normally suspended. It makes up about 55% of the total blood volume. It is the intravascular fluid part of extracellular fluid...
. The process was first commercialized with human albumin for medical use and later adopted for production of BSA.
Properties
The full-length BSA precursor protein is 607 amino acids in length. An N-terminal 18-residue signal peptideSignal peptide
A signal peptide is a short peptide chain that directs the transport of a protein.Signal peptides may also be called targeting signals, signal sequences, transit peptides, or localization signals....
is cut off from the precursor protein upon secretion, hence the initial protein product contains 589 amino acid residues. An additional 4 amino acids is cleaved to yield the mature BSA protein that contains 585 amino acids.
| Peptide | Position | Length | MW Da Atomic mass unit The unified atomic mass unit or dalton is a unit that is used for indicating mass on an atomic or molecular scale. It is defined as one twelfth of the rest mass of an unbound neutral atom of carbon-12 in its nuclear and electronic ground state, and has a value of... |
|---|---|---|---|
| Full length precursor | 1 – 607 | 607 | 69,324 |
| Signal peptide Signal peptide A signal peptide is a short peptide chain that directs the transport of a protein.Signal peptides may also be called targeting signals, signal sequences, transit peptides, or localization signals.... |
1 – 18 | 18 | 2,107 |
| Propeptide | 19 – 22 | 4 | 478 |
| Mature protein | 25 – 607 | 583 | 66,463 |
Physical properties of BSA:
- Number of amino acid residues: 585
- Molecular weight: 66,463 DaAtomic mass unitThe unified atomic mass unit or dalton is a unit that is used for indicating mass on an atomic or molecular scale. It is defined as one twelfth of the rest mass of an unbound neutral atom of carbon-12 in its nuclear and electronic ground state, and has a value of...
(= 66.5 kDa) - isoelectric pointIsoelectric pointThe isoelectric point , sometimes abbreviated to IEP, is the pH at which a particular molecule or surface carries no net electrical charge....
in water at 25 °C: 4.7 - Extinction coefficient of 43,824 M−1cm−1 at 279 nm
- Dimensions: 140 X 40 X 40 Å3 (prolate ellipsoid where a = b < c)
Applications
BSA has numerous biochemical applications including ELISAELISA
Enzyme-linked immunosorbent assay , is a popular format of a "wet-lab" type analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay to detect the presence of a substance in a liquid sample."Wet lab" analytic biochemistry assays involves detection of an...
s (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry
Immunohistochemistry
Immunohistochemistry or IHC refers to the process of detecting antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC takes its name from the roots "immuno," in reference to antibodies used in the procedure, and...
. It is also used as a nutrient in cell and microbial culture. In restriction digest
Restriction digest
A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation...
s, BSA is used to stabilize some enzymes during digestion
Digestion
Digestion is the mechanical and chemical breakdown of food into smaller components that are more easily absorbed into a blood stream, for instance. Digestion is a form of catabolism: a breakdown of large food molecules to smaller ones....
of DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...
and to prevent adhesion of the enzyme to reaction tubes and other vessels
Blood vessel
The blood vessels are the part of the circulatory system that transports blood throughout the body. There are three major types of blood vessels: the arteries, which carry the blood away from the heart; the capillaries, which enable the actual exchange of water and chemicals between the blood and...
. This protein
Protein
Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form, facilitating a biological function. A polypeptide is a single linear polymer chain of amino acids bonded together by peptide bonds between the carboxyl and amino groups of...
does not affect other enzyme
Enzyme
Enzymes are proteins that catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates...
s that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA (see Bradford protein assay
Bradford protein assay
The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M...
). BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.
See also
- Acceptable daily intakeAcceptable daily intakeAcceptable daily intake or ADI is a measure of the amount of a specific substance in food or drinking water that can be ingested on a daily basis over a lifetime without an appreciable health risk...
- Protein allergy
- Human serum albuminHuman serum albuminHuman serum albumin is the most abundant protein in human blood plasma. It is produced in the liver. Albumin constitutes about half of the blood serum protein...
- Serum albuminSerum albuminSerum albumin, often referred to simply as albumin is a protein that in humans is encoded by the ALB gene.Serum albumin is the most abundant plasma protein in mammals. Albumin is essential for maintaining the osmotic pressure needed for proper distribution of body fluids between intravascular...