ANNINE-6plus
Encyclopedia
ANNINE-6plus is a water soluble voltage sensitive dye
(also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmember voltage of excitable cells, including neurons, skeletal and cardiac myocytes.
heart. Using confocal microscopy
in conjunction with ANNINE-6plus, single sweep action potentials with high peak signal-to-noise ratio
(SNR) have been recorded from single transverse tubule (t-tubule
) of a few micrometers in the ventricular
cardiomyocyte.
Voltage sensitive dye
Voltage-sensitive dyes, also known as potentiometric dyes, are dyes which change their spectral properties in response to voltage changes. They are able to provide linear measurements of firing activity of single neurons, large neuronal populations or activity of myocytes...
(also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmember voltage of excitable cells, including neurons, skeletal and cardiac myocytes.
Voltage sensitivity
ANNINE-6plus has a fractional fluorescent intensity change (ΔF/F per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm).Applications
ANNINE-6plus has been applied in the microscopic imaging of action potentials of cardiomyocyte in perfused miceMICE
-Fiction:*Mice , alien species in The Hitchhiker's Guide to the Galaxy*The Mice -Acronyms:* "Meetings, Incentives, Conferencing, Exhibitions", facilities terminology for events...
heart. Using confocal microscopy
Confocal microscopy
Confocal microscopy is an optical imaging technique used to increase optical resolution and contrast of a micrograph by using point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane. It enables the reconstruction of...
in conjunction with ANNINE-6plus, single sweep action potentials with high peak signal-to-noise ratio
Signal-to-noise ratio
Signal-to-noise ratio is a measure used in science and engineering that compares the level of a desired signal to the level of background noise. It is defined as the ratio of signal power to the noise power. A ratio higher than 1:1 indicates more signal than noise...
(SNR) have been recorded from single transverse tubule (t-tubule
T-tubule
A T-tubule is a deep invagination of the sarcolemma, which is the plasma membrane, only found in skeletal and cardiac muscle cells...
) of a few micrometers in the ventricular
Ventricle (heart)
In the heart, a ventricle is one of two large chambers that collect and expel blood received from an atrium towards the peripheral beds within the body and lungs. The Atria primes the Pump...
cardiomyocyte.