Spin trapping
Encyclopedia
Spin trapping in chemistry
is an analytical technique employed in the detection and identification of short-lived free radicals. Spin trapping involves the addition of radical to a nitrone
spin trap resulting in the formation of a spin adduct, a nitroxide-based persistent radical, that can be detected using electron paramagnetic resonance
(EPR) spectroscopy. The spin adduct usually yields a distinctive EPR spectrum characteristic of a particular free radical that is trapped. The identity of the radical can be inferred based from the EPR spectral profile of their respective spin adducts such as the g value, but most importantly, the hyperfine-coupling constants of relevant nuclei. Unambiguous assignments of the identity of the trapped radical can often be made by using stable isotope substitution of the radicals parent compound, so that further hyperfine couplings are introduced or altered.
The most commonly used spin traps are alpha-phenyl N-tertiary-butyl nitrone (PBN) and 5,5-dimethyl-pyrroline N-oxide (DMPO). More rarely, C-nitroso spin traps such as 3,5-Dibromo-4-nitrosobenzenesulfonic acid (DBNBS) can be used as spin traps: often additional hyperfine information is derived, but at a cost of specificity (due to facile non-radical addition of many compounds to C-nitroso species, and subsequent oxidation of the resulting hydroxylamine).
5-Diisopropoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DIPPMPO) spin trapping has been used in measuring superoxide production in mitochondria.
The use of radical-addition reactions to detect short-lived radicals was first proposed by E. G. Janzen in 1965.
It is worth noting that the radical adduct (or products such as the hydroxylamine) can often be stable enough to allow non-EPR detection techniques. The groups of London, and Berliner & Khrahmtsov have used NMR to study such adducts and Timmins and co-workers used charge changes upon DBNBS trapping to isolate protein adducts for study. A major advance has been the development of anti-DMPO antibodies by Mason's group, allowing study of spin trapping reactions by a simple immuno-based techniques.
Chemistry
Chemistry is the science of matter, especially its chemical reactions, but also its composition, structure and properties. Chemistry is concerned with atoms and their interactions with other atoms, and particularly with the properties of chemical bonds....
is an analytical technique employed in the detection and identification of short-lived free radicals. Spin trapping involves the addition of radical to a nitrone
Nitrone
A nitrone is the N-oxide of an imine and a functional group in organic chemistry. The general structure is R1R2C=NR3+O- where R3 is different from H.A nitrone is 1,3-dipole in 1,3-dipolar cycloadditions...
spin trap resulting in the formation of a spin adduct, a nitroxide-based persistent radical, that can be detected using electron paramagnetic resonance
Electron paramagnetic resonance
Electron paramagnetic resonance or electron spin resonance spectroscopyis a technique for studying chemical species that have one or more unpaired electrons, such as organic and inorganic free radicals or inorganic complexes possessing a transition metal ion...
(EPR) spectroscopy. The spin adduct usually yields a distinctive EPR spectrum characteristic of a particular free radical that is trapped. The identity of the radical can be inferred based from the EPR spectral profile of their respective spin adducts such as the g value, but most importantly, the hyperfine-coupling constants of relevant nuclei. Unambiguous assignments of the identity of the trapped radical can often be made by using stable isotope substitution of the radicals parent compound, so that further hyperfine couplings are introduced or altered.
The most commonly used spin traps are alpha-phenyl N-tertiary-butyl nitrone (PBN) and 5,5-dimethyl-pyrroline N-oxide (DMPO). More rarely, C-nitroso spin traps such as 3,5-Dibromo-4-nitrosobenzenesulfonic acid (DBNBS) can be used as spin traps: often additional hyperfine information is derived, but at a cost of specificity (due to facile non-radical addition of many compounds to C-nitroso species, and subsequent oxidation of the resulting hydroxylamine).
5-Diisopropoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DIPPMPO) spin trapping has been used in measuring superoxide production in mitochondria.
The use of radical-addition reactions to detect short-lived radicals was first proposed by E. G. Janzen in 1965.
It is worth noting that the radical adduct (or products such as the hydroxylamine) can often be stable enough to allow non-EPR detection techniques. The groups of London, and Berliner & Khrahmtsov have used NMR to study such adducts and Timmins and co-workers used charge changes upon DBNBS trapping to isolate protein adducts for study. A major advance has been the development of anti-DMPO antibodies by Mason's group, allowing study of spin trapping reactions by a simple immuno-based techniques.