Seidel Test
Encyclopedia
The Seidel Test is used to assess the presence of anterior chamber
Anterior chamber
The anterior chamber is the fluid-filled space inside the eye between the iris and the cornea's innermost surface, the endothelium. Aqueous humor is the fluid that fills the anterior chamber. Hyphema and glaucoma are two main pathologies in this area. In hyphema, blood fills the anterior chamber...

 leakage in the cornea
Cornea
The cornea is the transparent front part of the eye that covers the iris, pupil, and anterior chamber. Together with the lens, the cornea refracts light, with the cornea accounting for approximately two-thirds of the eye's total optical power. In humans, the refractive power of the cornea is...

. It is used as a screening test for many corneal disorders including corneal post-trauma, corneal perforation
Corneal perforation
Corneal Perforation is an anomaly in the cornea resulting from damage to the corneal surface. A corneal perforation means that the cornea has been penetrated, thus leaving the cornea damaged....

 and corneal degeneration.

Procedure

A fluorescein
Fluorescein
Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol. It is widely used as a fluorescent tracer for many applications....

 strip containing 10% fluorescein is applied topically to the affected area and is examined with a cobalt blue
Cobalt blue
Cobalt blue is a cool, slightly desaturated blue color, historically made using cobalt salts of alumina. It is used in certain ceramics and painting; the different cobalt pigment smalt, based on silica, is more often used directly in tinted transparent glasses...

filter. At this point, the fluorescein appears dark orange in color. Any changes in color of the fluorescein strip indicate the presence of corneal deformities.

Results of the test

If the fluorescein strip turns pale upon application to the corneal surface, the person tests positive for the corneal deformity he/ she is being tested for. The change in the color of the fluorescein strip is due to dilution of fluorescein caused by the aqueous leakage in the cornea.

Precautions

Do not apply pressure on the eye during the test due to the risk of tissue extrusion.

See also


External links

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