Second Generation Multiplex
Encyclopedia
Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up
the UK National DNA Database
in 1995. It is manufactured by ABI (Applied Biosystems).
It contains primers for the following STR (Short Tandem Repeat
) loci.
VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), FGA (HUMFIBRA)
Also contains primers for the Amelogenin
sex indicating test.
The primers are tagged with the following fluorescent dyes for detection under electrophoresis.
Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database
was superseded by the Second Generation Multiplex Plus
SGM+ DNA profiling system in 1998
the UK National DNA Database
UK National DNA Database
The United Kingdom National DNA Database is a national DNA Database that was set up in 1995. As of the end of 2005, it carried the profiles of around 3.1 million people...
in 1995. It is manufactured by ABI (Applied Biosystems).
It contains primers for the following STR (Short Tandem Repeat
Short tandem repeat
A short tandem repeat in DNA occurs when a pattern of two or more nucleotides are repeated and the repeated sequences are directly adjacent to each other. The pattern can range in length from 2 to 5 base pairs and is typically in the non-coding intron region...
) loci.
VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), FGA (HUMFIBRA)
Also contains primers for the Amelogenin
Amelogenin
Amelogenin is a protein found in developing tooth enamel, and it belongs to a family of extracellular matrix proteins. Developing enamel contains about 30% protein, and 90% of this is amelogenins...
sex indicating test.
The primers are tagged with the following fluorescent dyes for detection under electrophoresis.
- 5-FAMCarboxyfluoresceinCarboxyfluorescein refers to two fluorescent dyes with an excitation and emission of 492/517 nm, respectively. They are commonly used as a tracer agents. The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. It can be incorporated into liposomes,...
- JOE
- NED
Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database
UK National DNA Database
The United Kingdom National DNA Database is a national DNA Database that was set up in 1995. As of the end of 2005, it carried the profiles of around 3.1 million people...
was superseded by the Second Generation Multiplex Plus
Second Generation Multiplex Plus
Second Generation Multiplex Plus , is a DNA profiling system developed by Applied Biosystems. It is an updated version of Second Generation Multiplex...
SGM+ DNA profiling system in 1998