Carboxyfluorescein
Encyclopedia
Carboxyfluorescein refers to two fluorescent dye
s with an excitation and emission of 492/517 nm, respectively. They are commonly used as a tracer agents. The dyes are membrane-impermeant and can be loaded into cells by microinjection
or scrape loading. It can be incorporated into liposomes, and allow for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells.
Dye
A dye is a colored substance that has an affinity to the substrate to which it is being applied. The dye is generally applied in an aqueous solution, and requires a mordant to improve the fastness of the dye on the fiber....
s with an excitation and emission of 492/517 nm, respectively. They are commonly used as a tracer agents. The dyes are membrane-impermeant and can be loaded into cells by microinjection
Microinjection
Microinjection refers to the process of using a glass micropipette to insert substances at a microscopic or borderline macroscopic level into a single living cell. It is a simple mechanical process in which a needle roughly 0.5 to 5 micrometers in diameter penetrates the cell membrane and/or the...
or scrape loading. It can be incorporated into liposomes, and allow for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells.