Replication factor C
Encyclopedia
The replication factor C, or RFC, is a five-subunit protein complex
that is required for DNA replication
.
The subunits
of this heteropentamer are named Rfc1
, Rfc2
, Rfc3
, Rfc4
, and Rfc5
(in S. cerevisiae). RFC is used in eukaryotic replication as a clamp loader, similar to the γ Complex in E. coli. Its role as clamp loader involves catalysing the loading of PCNA on to DNA. It binds to the 3' end of the DNA and uses ATP hydrolysis to open the ring of PCNA so that it can encircle the DNA.
Protein complex
A multiprotein complex is a group of two or more associated polypeptide chains. If the different polypeptide chains contain different protein domain, the resulting multiprotein complex can have multiple catalytic functions...
that is required for DNA replication
DNA replication
DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for biological inheritance. The process starts with one double-stranded DNA molecule and produces two identical copies of the molecule...
.
The subunits
Protein subunit
In structural biology, a protein subunit or subunit protein is a single protein molecule that assembles with other protein molecules to form a protein complex: a multimeric or oligomeric protein. Many naturally occurring proteins and enzymes are multimeric...
of this heteropentamer are named Rfc1
RFC1
Replication factor C subunit 1 is a protein that in humans is encoded by the RFC1 gene.-Interactions:RFC1 has been shown to interact with BRD4, HDAC1, RFC3, RELA and PCNA.-Further reading:...
, Rfc2
RFC2
Replication factor C subunit 2 is a protein that in humans is encoded by the RFC2 gene.-Interactions:RFC2 has been shown to interact with BRD4, CHTF18, PCNA, RFC4 and RFC5.-Further reading:...
, Rfc3
RFC3
Replication factor C subunit 3 is a protein that in humans is encoded by the RFC3 gene.-Interactions:RFC3 has been shown to interact with BRD4, CHTF18, RFC1, PCNA and RFC4.-Further reading:...
, Rfc4
RFC4
Replication factor C subunit 4 is a protein that in humans is encoded by the RFC4 gene.-Interactions:RFC4 has been shown to interact with BRD4, RFC2, RFC3, CHTF18, PCNA and RFC5.-Further reading:...
, and Rfc5
RFC5
Replication factor C subunit 5 is a protein that in humans is encoded by the RFC5 gene.-Interactions:RFC5 has been shown to interact with BRD4, RFC2, PCNA, RFC4 and CHTF18.-Further reading:...
(in S. cerevisiae). RFC is used in eukaryotic replication as a clamp loader, similar to the γ Complex in E. coli. Its role as clamp loader involves catalysing the loading of PCNA on to DNA. It binds to the 3' end of the DNA and uses ATP hydrolysis to open the ring of PCNA so that it can encircle the DNA.