Histidine-tryptophan-ketoglutarate
Encyclopedia
Histidine-tryptophan-ketoglutarate, or Custodiol HTK solution is a high-flow, low-potassium
preservation solution used for organ transplantation.
HTK solution is intended for perfusion
and flushing of donor liver kidney, heart, lung and pancreas prior to removal from the donor and for preserving these organs during hypothermic storage and transport to the recipient. HTK solution is based on the principle of inactivating organ function by withdrawal of extracellular
sodium
and calcium
, together with intensive buffering of the extracellular space by means of histidine
/histidine hydrochloride, so as to prolong the period during which the organs will tolerate interruption of oxygenated blood. The composition of HTK is similar to that of extracellular fluid
. All of the components of HTK occur naturally in the body.
Custodiol HTK is the registered trademark of Dr. Franz Köhler (Chemie GmbH, Germany).
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Potassium
Potassium is the chemical element with the symbol K and atomic number 19. Elemental potassium is a soft silvery-white alkali metal that oxidizes rapidly in air and is very reactive with water, generating sufficient heat to ignite the hydrogen emitted in the reaction.Potassium and sodium are...
preservation solution used for organ transplantation.
HTK solution is intended for perfusion
Perfusion
In physiology, perfusion is the process of nutritive delivery of arterial blood to a capillary bed in the biological tissue. The word is derived from the French verb "perfuser" meaning to "pour over or through."...
and flushing of donor liver kidney, heart, lung and pancreas prior to removal from the donor and for preserving these organs during hypothermic storage and transport to the recipient. HTK solution is based on the principle of inactivating organ function by withdrawal of extracellular
Extracellular
In cell biology, molecular biology and related fields, the word extracellular means "outside the cell". This space is usually taken to be outside the plasma membranes, and occupied by fluid...
sodium
Sodium
Sodium is a chemical element with the symbol Na and atomic number 11. It is a soft, silvery-white, highly reactive metal and is a member of the alkali metals; its only stable isotope is 23Na. It is an abundant element that exists in numerous minerals, most commonly as sodium chloride...
and calcium
Calcium
Calcium is the chemical element with the symbol Ca and atomic number 20. It has an atomic mass of 40.078 amu. Calcium is a soft gray alkaline earth metal, and is the fifth-most-abundant element by mass in the Earth's crust...
, together with intensive buffering of the extracellular space by means of histidine
Histidine
Histidine Histidine, an essential amino acid, has a positively charged imidazole functional group. It is one of the 22 proteinogenic amino acids. Its codons are CAU and CAC. Histidine was first isolated by German physician Albrecht Kossel in 1896. Histidine is an essential amino acid in humans...
/histidine hydrochloride, so as to prolong the period during which the organs will tolerate interruption of oxygenated blood. The composition of HTK is similar to that of extracellular fluid
Extracellular fluid
Extracellular fluid usually denotes all body fluid outside of cells. The remainder is called intracellular fluid.In some animals, including mammals, the extracellular fluid can be divided into two major subcompartments, interstitial fluid and blood plasma...
. All of the components of HTK occur naturally in the body.
| Component | HTK |
|---|---|
| Sodium (mmol/L) | 15 |
| Potassium (mmol/L) | 10 |
| Magnesium (mmol/L) | 4 |
| Calcium (mmol/L) | 0.015 |
| Ketoglutarate/glutamic acid (mmol/L) | 1 |
| Histidine (mmol/L) | 198 |
| Mannitol (mmol/L) | 30 |
| Tryptophan (mmol/L) | 2 |
| Osmolarity (mOsm/L) | 310 |
Custodiol HTK is the registered trademark of Dr. Franz Köhler (Chemie GmbH, Germany).
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