Higher-energy C-trap dissociation
Encyclopedia
Higher-energy collisional dissociation (HCD, formerly higher-energy C-trap dissociation) is a fragmentation
technique, often used for peptide
modification
analysis. Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. An added octopole collision cell facilitates de novo sequencing.
Fragmentation (chemistry)
Fragmentation is a type of chemical dissociation. Fragmentation of a molecule can take place by a process of heterolysis or homolysis.It is a phenomenon observed in mass spectrometry where it is used as a tool to find the structural formula of a molecule, process called structural elucidation.It...
technique, often used for peptide
Peptide
Peptides are short polymers of amino acid monomers linked by peptide bonds. They are distinguished from proteins on the basis of size, typically containing less than 50 monomer units. The shortest peptides are dipeptides, consisting of two amino acids joined by a single peptide bond...
modification
Posttranslational modification
Posttranslational modification is the chemical modification of a protein after its translation. It is one of the later steps in protein biosynthesis, and thus gene expression, for many proteins....
analysis. Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. An added octopole collision cell facilitates de novo sequencing.