Enterovirus cis-acting replication element
Encyclopedia
Enterovirus cis-acting replication element is a small RNA
hairpin
in the coding region of protein 2C as the site in PV1(M) RNA that is used as the primary template for the in vitro
uridylylation. The first step in the replication of the plus-stranded poliovirus
RNA is the synthesis of a complementary minus strand. This process is initiated by the covalent attachment of Uridine monophosphate
(UMP) to the terminal protein VPg
, yielding VPgpU and VPgpUpU.
RNA
Ribonucleic acid , or RNA, is one of the three major macromolecules that are essential for all known forms of life....
hairpin
Stem-loop
Stem-loop intramolecular base pairing is a pattern that can occur in single-stranded DNA or, more commonly, in RNA. The structure is also known as a hairpin or hairpin loop. It occurs when two regions of the same strand, usually complementary in nucleotide sequence when read in opposite directions,...
in the coding region of protein 2C as the site in PV1(M) RNA that is used as the primary template for the in vitro
In vitro
In vitro refers to studies in experimental biology that are conducted using components of an organism that have been isolated from their usual biological context in order to permit a more detailed or more convenient analysis than can be done with whole organisms. Colloquially, these experiments...
uridylylation. The first step in the replication of the plus-stranded poliovirus
Poliovirus
Poliovirus, the causative agent of poliomyelitis, is a human enterovirus and member of the family of Picornaviridae.Poliovirus is composed of an RNA genome and a protein capsid. The genome is a single-stranded positive-sense RNA genome that is about 7500 nucleotides long. The viral particle is...
RNA is the synthesis of a complementary minus strand. This process is initiated by the covalent attachment of Uridine monophosphate
Uridine monophosphate
Uridine monophosphate, also known as 5'-uridylic acid and abbreviated UMP, is a nucleotide that is used as a monomer in RNA. It is an ester of phosphoric acid with the nucleoside uridine...
(UMP) to the terminal protein VPg
VPg
VPg is a protein attached to the 5' end of RNA during RNA synthesis in a wide variety of viruses including Picornaviridae such as Foot-and-mouth disease and poliovirus . VPg stands for "viral protein genome-linked".-Attachment during RNA synthesis:...
, yielding VPgpU and VPgpUpU.
See also
- Enteroviral 3' UTR elementEnteroviral 3' UTR elementIn molecular biology, the enteroviral 3' UTR element is an RNA structure found in the 3' UTR of various enteroviruses. The overall structure forms the origin of replication for the initiation of strand RNA synthesis...
- Enterovirus 5' cloverleaf cis-acting replication elementEnterovirus 5' cloverleaf cis-acting replication elementThe Enterovirus 5' cloverleaf cis-acting replication element is an RNA element found in the 5' UTR of Enterovirus genomes. The element has a cloverleaf like secondary structure and is known to be a multifunctional cis-acting replication element , required for the initiation of negative strand RNA...