Cresol Red
Encyclopedia
Cresol Red is a triarylmethane dye
Triarylmethane dye
Triarylmethane dyes are synthetic organic compounds containing triphenylmethane backbones. As dyes, these compounds are intensely colored. Many of these dyes undergo reactions in response to acid and base, and thus serve as pH indicators....

 frequently used for monitoring the pH
PH
In chemistry, pH is a measure of the acidity or basicity of an aqueous solution. Pure water is said to be neutral, with a pH close to 7.0 at . Solutions with a pH less than 7 are said to be acidic and solutions with a pH greater than 7 are basic or alkaline...

 in aquaria
Aquarium
An aquarium is a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept. Fishkeepers use aquaria to keep fish, invertebrates, amphibians, marine mammals, turtles, and aquatic plants...

.

pH indicator













Molecular biology

Cresol Red can be used in many common molecular biology reactions in place of other loading dyes. Cresol Red does not inhibit Taq polymerase to the same degree as other common loading dyes.

Color marker

Cresol Red can also be used as a color marker
Color marker
A Color marker is used to monitor the progress of agarose gel electrophoresis and polyacrylamide gel electrophoresis since DNA, RNA, and most proteins are colourless. They are also referred to as tracking dyes....

 to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel it runs approximately at the size of a 125 base pair (bp) DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...

 molecule (it depends on the concentration of buffer and other component). Bromophenol blue
Bromophenol blue
Bromophenol blue is used as an acid-base indicator, a color marker and a dye.-Acid-base indicator:As an acid-base indicator its useful range lies between pH 3.0 and 4.6...

 and xylene cyanol
Xylene cyanol
Xylene cyanol can be used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Bromophenol blue and orange G can also be used for this purpose.-Migration speed:...

can also be used for this purpose.

External links

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