Upstream Activating Sequence
Encyclopedia
An UAS is a cis-acting regulatory sequence
. It is distinct from the promoter and increases the expression
of a neighbouring gene
. Its property to bind the GAL4 protein is utilised in the GAL4/UAS technique
for controlled gene misexpression in Drosophila.
) eventually after growing for hours in YPD (Yeast peptone dextrose) media. After using up glucose for fermentation, these genes get activated for aerobic respiration.
The Gal4 dimer binds to the UASs upstream of the various GAL genes (Cortes).
Cis-regulatory element
A cis-regulatory element or cis-element is a region of DNA or RNA that regulates the expression of genes located on that same molecule of DNA . This term is constructed from the Latin word cis, which means "on the same side as". These cis-regulatory elements are often binding sites for one or...
. It is distinct from the promoter and increases the expression
Transcription (genetics)
Transcription is the process of creating a complementary RNA copy of a sequence of DNA. Both RNA and DNA are nucleic acids, which use base pairs of nucleotides as a complementary language that can be converted back and forth from DNA to RNA by the action of the correct enzymes...
of a neighbouring gene
Gene
A gene is a molecular unit of heredity of a living organism. It is a name given to some stretches of DNA and RNA that code for a type of protein or for an RNA chain that has a function in the organism. Living beings depend on genes, as they specify all proteins and functional RNA chains...
. Its property to bind the GAL4 protein is utilised in the GAL4/UAS technique
GAL4/UAS system
The GAL4-UAS system is a biochemical method used to study gene expression and function in organisms such as the fruit fly. It was developed by Andrea Brand and Norbert Perrimon in 1993 and is considered a powerful technique for studying the expression of genes...
for controlled gene misexpression in Drosophila.
Instances
It was been shown to be involved in five of the seven genes which are glucose repressed. These genes were repressed in yeast (Saccharomyces cerevisiaeSaccharomyces cerevisiae
Saccharomyces cerevisiae is a species of yeast. It is perhaps the most useful yeast, having been instrumental to baking and brewing since ancient times. It is believed that it was originally isolated from the skin of grapes...
) eventually after growing for hours in YPD (Yeast peptone dextrose) media. After using up glucose for fermentation, these genes get activated for aerobic respiration.
The Gal4 dimer binds to the UASs upstream of the various GAL genes (Cortes).