U87
Encyclopedia
In cell biology
Cell biology
Cell biology is a scientific discipline that studies cells – their physiological properties, their structure, the organelles they contain, interactions with their environment, their life cycle, division and death. This is done both on a microscopic and molecular level...

 , U87 is a human glioblastoma cell line formally known as U-87 MG. It has epithelial morphology
Morphology (biology)
In biology, morphology is a branch of bioscience dealing with the study of the form and structure of organisms and their specific structural features....

, and was obtained from a stage three cancer patient.

U-87 MG can be obtained from the American Type Culture Collection
American Type Culture Collection
The American Type Culture Collection is a private, not-for-profit biological resource center whose mission focuses on the acquisition, authentication, production, preservation, development and distribution of standard reference microorganisms, cell lines and other materials for research in the...

 (ATCC) where it is known by the accession number HTB-14.

The entire sequence of the genome of U-87 MG has recently been published in PLoS Genetics, 2010 January; 6(1): e1000832.

Growth conditions

U87 growth media is generally made with DMEM + 10% FBS supplemented with 2 mM L-Glutamine and 1% non-essential amino acid
Amino acid
Amino acids are molecules containing an amine group, a carboxylic acid group and a side-chain that varies between different amino acids. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen...

s. It is propagated at 37 degrees Celsius in a 5% carbon dioxide atmosphere.

External links

The source of this article is wikipedia, the free encyclopedia.  The text of this article is licensed under the GFDL.
 
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