Turnip crinkle virus
Encyclopedia
Turnip crinkle virus is a plant pathogenic virus of the family Tombusviridae
.
TCV is a small (4054bp), icosohedral, single stranded, positive sense RNA virus (viral RNA is in the same orientation as mRNA). It has been shown to infect various types of plant species including the common plant model, Arabidopsis thaliana
, and is currently being studied in yeast. Its RNA encodes for five proteins: p28 and p88 (replication), p8 and p9 (movement) and CP (coat protein or encapsidation).
Replication of the viral RNA begins with the migration of p28 to the mitchondrial membrane. p28 invaginates the outer mitochondrial membrane in addition to a few p88 molecules (read-through product of p28). The viral RNA binds to the p28 bound to the membrane and the RNA dependent RNA polymerase, or p88, initiates replication of the positive strand RNA to produce a minus strand intermediate. The minus strand intermediate is used as a template to produce progeny positive strand RNA.
A number of non-coding RNA
elements have been characterised in the TCV genome; examples are hairpin 5
and the core promoter
.
Tombusviridae
Tombusviridae is a family of single-stranded positive sense RNA plant viruses. The name is derived from the type species of the Tombusvirus genus, Tomato bushy stunt virus...
.
TCV is a small (4054bp), icosohedral, single stranded, positive sense RNA virus (viral RNA is in the same orientation as mRNA). It has been shown to infect various types of plant species including the common plant model, Arabidopsis thaliana
Arabidopsis thaliana
Arabidopsis thaliana is a small flowering plant native to Europe, Asia, and northwestern Africa. A spring annual with a relatively short life cycle, arabidopsis is popular as a model organism in plant biology and genetics...
, and is currently being studied in yeast. Its RNA encodes for five proteins: p28 and p88 (replication), p8 and p9 (movement) and CP (coat protein or encapsidation).
Replication of the viral RNA begins with the migration of p28 to the mitchondrial membrane. p28 invaginates the outer mitochondrial membrane in addition to a few p88 molecules (read-through product of p28). The viral RNA binds to the p28 bound to the membrane and the RNA dependent RNA polymerase, or p88, initiates replication of the positive strand RNA to produce a minus strand intermediate. The minus strand intermediate is used as a template to produce progeny positive strand RNA.
A number of non-coding RNA
Non-coding RNA
A non-coding RNA is a functional RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA , non-messenger RNA and functional RNA . The term small RNA is often used for short bacterial ncRNAs...
elements have been characterised in the TCV genome; examples are hairpin 5
Turnip crinkle virus (TCV) repressor of minus strand synthesis H5
The TCV hairpin 5 is an RNA element found in the turnip crinkle virus. This RNA element is composed of a stem-loop that contains a large symmetrical internal loop . H5 can repress minus-strand synthesis when the 3' side of the LSL pairs with the 4 bases at the 3'-terminus of the RNA....
and the core promoter
Turnip crinkle virus (TCV) core promoter hairpin (Pr)
The turnip crinkle virus core promoter hairpin is an RNA element located in the 3' UTR of the viral genome that is required for minus strand RNA synthesis. The picture shown is not the TCV core promoter, but an upstream hairpin that is also required for replication of the virus....
.