Trizol
Encyclopedia
TRIzol is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from Piotr Chomczyński and Sacchi, N. in 1987.
TRIzol is the brand name of the product from Invitrogen
, and the brand name from MRC, which was founded by Chomczynski, is TRI Reagent.
The correct name of the method is Guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA and RNA yields comparable to other extraction methods. An alternative method for RNA extraction
is phenol extraction and TCA/acetone precipitation. Chloroform be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.
TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.
When used, it resembles cough syrup, bright pink. The smell of the phenol
and guanidinium thiocyanate is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIzol (specifically phenol
) and Chloroform
.
TRIzol is the brand name of the product from Invitrogen
Invitrogen
Invitrogen Corporation was a large, multinational biotechnology company headquartered in Carlsbad, California. In November 2008, a merger between Applied Biosystems and Invitrogen was finalized...
, and the brand name from MRC, which was founded by Chomczynski, is TRI Reagent.
The correct name of the method is Guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA and RNA yields comparable to other extraction methods. An alternative method for RNA extraction
RNA extraction
RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA...
is phenol extraction and TCA/acetone precipitation. Chloroform be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.
TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.
When used, it resembles cough syrup, bright pink. The smell of the phenol
Phenol
Phenol, also known as carbolic acid, phenic acid, is an organic compound with the chemical formula C6H5OH. It is a white crystalline solid. The molecule consists of a phenyl , bonded to a hydroxyl group. It is produced on a large scale as a precursor to many materials and useful compounds...
and guanidinium thiocyanate is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIzol (specifically phenol
Phenol
Phenol, also known as carbolic acid, phenic acid, is an organic compound with the chemical formula C6H5OH. It is a white crystalline solid. The molecule consists of a phenyl , bonded to a hydroxyl group. It is produced on a large scale as a precursor to many materials and useful compounds...
) and Chloroform
Chloroform
Chloroform is an organic compound with formula CHCl3. It is one of the four chloromethanes. The colorless, sweet-smelling, dense liquid is a trihalomethane, and is considered somewhat hazardous...
.