Sulforhodamine 101 is a red fluorescent dye

Dye

A dye is a colored substance that has an affinity to the substrate to which it is being applied. The dye is generally applied in an aqueous solution, and requires a mordant to improve the fastness of the dye on the fiber....

. In neurophysiological experiments which comprise calcium imaging

Calcium imaging

Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium status of a tissue or medium....

 methods, it can be used for a counterstain

Counterstain

A counterstain is a stain with color contrasting to the principal stain, making the stained structure more easily visible.An example is the malachite green counterstain to the fuchsine stain in the Gimenez staining technique....

ing of astrocyte

Astrocyte

Astrocytes , also known collectively as astroglia, are characteristic star-shaped glial cells in the brain and spinal cord...

s to be able to analyze data from neuron

Neuron

A neuron is an electrically excitable cell that processes and transmits information by electrical and chemical signaling. Chemical signaling occurs via synapses, specialized connections with other cells. Neurons connect to each other to form networks. Neurons are the core components of the nervous...

s separately.

A sulfonyl chloride derivative of sulforhodamine 101, known as Texas Red

Texas Red

Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.Texas Red fluoresces at about 615 nm,...

, is used for conjugation to a number of functional groups, especially primary amines.