Sudan Black B
Encyclopedia
Sudan Black B is a lysochrome
(fat-soluble dye) diazo dye used for staining
of neutral triglycerides and lipid
s on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and melting point 120-124 °C. It stains blue-black.
Sudan Black B is one of the dyes used for Sudan stain
ing.
Similar dyes include Oil Red O
, Sudan III
, and Sudan IV
.
Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.
A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.
In differentiating haematological
disorders Sudan black will stain myeloblasts but not lymphoblasts
.
Lysochrome
A lysochrome is a soluble dye used for biochemical staining of triglycerides, fatty acids, and lipoproteins. Lysochromes such as Sudan IV bind to the lipid in a substrate and show up as colored regions. The dye does not stick to any other substrates, so a quantification or qualification of lipid...
(fat-soluble dye) diazo dye used for staining
Staining (biology)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes...
of neutral triglycerides and lipid
Lipid
Lipids constitute a broad group of naturally occurring molecules that include fats, waxes, sterols, fat-soluble vitamins , monoglycerides, diglycerides, triglycerides, phospholipids, and others...
s on frozen sections and some lipoproteins on paraffin sections. It has the appearance of a dark brown to black powder with maximum absorption at 596-605 nm and melting point 120-124 °C. It stains blue-black.
Sudan Black B is one of the dyes used for Sudan stain
Sudan stain
Sudan staining is the use of Sudan dyes to stain sudanophilic substances, usually lipids. Sudan lysochromes are used....
ing.
Similar dyes include Oil Red O
Oil Red O
Oil Red O is a lysochrome diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections...
, Sudan III
Sudan III
Sudan III is a lysochrome diazo dye used for staining of triglycerides in frozen sections, and some protein bound lipids and lipoproteins on paraffin sections. It has the appearance of reddish brown crystals and a maximum absorption at 507 nm.Sudan III is a dye used for Sudan staining...
, and Sudan IV
Sudan IV
Sudan IV is a lysochrome diazo dye used for the staining of lipids, triglycerides and lipoproteins on frozen paraffin sections. It has the appearance of reddish brown crystals with melting point 199 °C and maximum absorption at 520 nm.Sudan IV is one of the dyes used for Sudan staining. Similar...
.
Sudan Black B can be used to stain some other materials than the other Sudan dyes, as it is not so specific to lipids.
A use of Sudan Black B is in fingerprint enhancement. It is useful for detecting fats that are contaminated with oil and grease.
In differentiating haematological
Hematological malignancy
Hematological malignancies are the types of cancer that affect blood, bone marrow, and lymph nodes. As the three are intimately connected through the immune system, a disease affecting one of the three will often affect the others as well: although lymphoma is technically a disease of the lymph...
disorders Sudan black will stain myeloblasts but not lymphoblasts
Acute lymphoblastic leukemia
Acute lymphoblastic leukemia is a form of leukemia, or cancer of the white blood cells characterized by excess lymphoblasts.Malignant, immature white blood cells continuously multiply and are overproduced in the bone marrow. ALL causes damage and death by crowding out normal cells in the bone...
.
External links
- Stains File entry