Sephadex
Encyclopedia
Sephadex is a trademark
for cross-linked
dextran
gel
used for gel filtration
. It was launched by Pharmacia
in 1959, after development work by Jerker Porath
and Per Flodin. The name is derived from separation Pharmacia dextran. It is normally manufactured in a bead form and most commonly used for gel filtration
columns. By varying the degree of cross-linking, the fractionation properties of the gel can be altered.
These highly specialized gel filtration and chromatographic
media are composed of macroscopic beads synthetically derived from the polysaccharide, dextran. The organic chains are cross-linked to give a three dimensional network having functional ionic groups attached by ether linkages to glucose units of the polysaccharide chains.
Available forms include anion and cation exchangers, as well as gel filtration resins, with varying degrees of porosity; bead sizes fall in discrete ranges between 20 and 300 µm
.
Trademark
A trademark, trade mark, or trade-mark is a distinctive sign or indicator used by an individual, business organization, or other legal entity to identify that the products or services to consumers with which the trademark appears originate from a unique source, and to distinguish its products or...
for cross-linked
Cross-link
Cross-links are bonds that link one polymer chain to another. They can be covalent bonds or ionic bonds. "Polymer chains" can refer to synthetic polymers or natural polymers . When the term "cross-linking" is used in the synthetic polymer science field, it usually refers to the use of...
dextran
Dextran
Dextran is a complex, branched glucan composed of chains of varying lengths...
gel
Gel
A gel is a solid, jelly-like material that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady-state...
used for gel filtration
Size exclusion chromatography
Size-exclusion chromatography is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight . It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers...
. It was launched by Pharmacia
Pharmacia
Pharmacia was a pharmaceutical and biotechnological company in Sweden.-History:Pharmacia was founded in 1911 in Stockholm, Sweden by pharmacist Gustav Felix Grönfeldt at the Elgen Pharmacy. The company is named after the Greek word φαρμακεία, transliterated pharmakeia, which means 'sorcery'...
in 1959, after development work by Jerker Porath
Jerker Porath
Jerker Porath, born 23 October 1921 in Sala, is a Swedish biochemist who has invented several separation methods for biomolecules.Porath studied at Uppsala University and initially did research in organic chemistry under Arne Fredga, where he got his licentiate degree...
and Per Flodin. The name is derived from separation Pharmacia dextran. It is normally manufactured in a bead form and most commonly used for gel filtration
Size exclusion chromatography
Size-exclusion chromatography is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight . It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers...
columns. By varying the degree of cross-linking, the fractionation properties of the gel can be altered.
These highly specialized gel filtration and chromatographic
Chromatography
Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures....
media are composed of macroscopic beads synthetically derived from the polysaccharide, dextran. The organic chains are cross-linked to give a three dimensional network having functional ionic groups attached by ether linkages to glucose units of the polysaccharide chains.
Available forms include anion and cation exchangers, as well as gel filtration resins, with varying degrees of porosity; bead sizes fall in discrete ranges between 20 and 300 µm
Micrometre
A micrometer , is by definition 1×10-6 of a meter .In plain English, it means one-millionth of a meter . Its unit symbol in the International System of Units is μm...
.