Second-order conditioning
Encyclopedia
In classical conditioning
Classical conditioning
Classical conditioning is a form of conditioning that was first demonstrated by Ivan Pavlov...

, second-order conditioning or higher-order conditioning is a form of learning
Learning
Learning is acquiring new or modifying existing knowledge, behaviors, skills, values, or preferences and may involve synthesizing different types of information. The ability to learn is possessed by humans, animals and some machines. Progress over time tends to follow learning curves.Human learning...

 in which a stimulus
Stimulation
Stimulation is the action of various agents on nerves, muscles, or a sensory end organ, by which activity is evoked; especially, the nervous impulse produced by various agents on nerves, or a sensory end organ, by which the part connected with the nerve is thrown into a state of activity.The word...

 is first made meaningful or consequential for an organism through an initial step of learning, and then that stimulus is used as a basis for learning about some new stimulus. For example, an animal might first learn to associate a bell with food (first-order conditioning), but then learn to associate a light with the bell (second-order conditioning). Honeybees show second-order conditioning during proboscis extension reflex
Proboscis extension reflex
Proboscis extension reflex is when a bee extends her proboscis as a reflex to antennal stimulation. It is evoked when a sugar solution is touched to a bee's antenna.- Use of PER :...

 conditioning.
Third order conditioning can then follow with a further stimulus being added and the response elicited can be weak. Also known as shaping behavior. Often related to B. F. Skinner
B. F. Skinner
Burrhus Frederic Skinner was an American behaviorist, author, inventor, baseball enthusiast, social philosopher and poet...

's studies with pigeons. Another example would be Skinner first conditioned a pigeon to walk up to a ball, then conditioning it to touch the ball with its beak. He would thus use a second-order to shape the animal's behavior. Often used with biological predispositions.

In Fear Conditioning

It has been demonstrated in an associative fear conditioning chain, such as CS2 --> CS1 --> US, that extinction
Extinction (psychology)
Extinction is the conditioning phenomenon in which a previously learned response to a cue is reduced when the cue is presented in the absence of the previously paired aversive or appetitive stimulus.-Fear conditioning:...

 of freezing responses to the first-order stimulus (CS1) leads to responding impairments in CS2, but extinction of the second-order stimulus (CS2), does not have any effect on CS1 (Debiec et al.). In the same study, the effect of activation (memory retrieval) on such an associative chain has been examined. Results demonstrated that protein synthesis inhibition after exposure to a single CS1 impairs responses to both CS1 and CS2, but protein synthesis inhibition after exposure to a single CS2, only disrupts CS2 and leaves CS1 freezing intact. Therefore, it is believed that when the first-order association is directly activated, it is placed into a labile state (as we would expect from reconsolidation research) which may have an impact on dependent associations. However, when the first-order association is only indirectly activated (through the associative chain), it appears that there is not sufficient stimulation to kick off cellular processes which would place it in a labile state, so it remains fixed.
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