Roswell Park Memorial Institute medium
Encyclopedia
Roswell Park Memorial Institute medium, commonly referred to as RPMI, is a form of medium
used in cell culture
and tissue culture. It has traditionally been used for growth of Human
lymphoid cells. This medium contains a great deal of phosphate
and is formulated for use in a 5% carbon dioxide
atmosphere.(RPMI) 1640, has traditionally been used for the serum-free expansion of human lymphoid cells.
RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.
Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells and hybridoma cells.
There are a variety of similar media in the RPMI series, such as RPMI 1640.
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI.html
Growth medium
A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens.There are different types of media for growing different types of cells....
used in cell culture
Cell culture
Cell culture is the complex process by which cells are grown under controlled conditions. In practice, the term "cell culture" has come to refer to the culturing of cells derived from singlecellular eukaryotes, especially animal cells. However, there are also cultures of plants, fungi and microbes,...
and tissue culture. It has traditionally been used for growth of Human
Human
Humans are the only living species in the Homo genus...
lymphoid cells. This medium contains a great deal of phosphate
Phosphate
A phosphate, an inorganic chemical, is a salt of phosphoric acid. In organic chemistry, a phosphate, or organophosphate, is an ester of phosphoric acid. Organic phosphates are important in biochemistry and biogeochemistry or ecology. Inorganic phosphates are mined to obtain phosphorus for use in...
and is formulated for use in a 5% carbon dioxide
Carbon dioxide
Carbon dioxide is a naturally occurring chemical compound composed of two oxygen atoms covalently bonded to a single carbon atom...
atmosphere.(RPMI) 1640, has traditionally been used for the serum-free expansion of human lymphoid cells.
RPMI 1640 uses a bicarbonate buffering system and differs from most mammalian cell culture media in its typical pH 8 formulation.
Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human T/B-lymphocytes, bone marrow cells and hybridoma cells.
There are a variety of similar media in the RPMI series, such as RPMI 1640.
External links
http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Product_Lines/Classic_Media___Salts/RPMI_Media.htmlhttp://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI.html