Rare-cutter enzyme
Encyclopedia
A rare-cutter enzyme is a restriction enzyme
with a recognition sequence
which occurs only rarely in a genome. An example is NotI, which cuts after the first GC of a 5'-GCGGCCGC-3' sequence; restriction enzymes with seven and eight base pair recognition sequences are often also called rare-cutter enzymes (six bp recognition sequences are much more common).
For example, rare-cutter enzymes with 7-nucleotide recognition sites cut once every 47 bp (16,384 bp), and those with 8-nucleotide recognition sites cut every 48 bp (65,536 bp) respectively. They are used in top-down mapping
to cut a chromosome into chunks of these sizes on average.
Restriction enzyme
A Restriction Enzyme is an enzyme that cuts double-stranded DNA at specific recognition nucleotide sequences known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses...
with a recognition sequence
Recognition sequence
The recognition sequence, sometimes also referred to as recognition site, of any DNA-binding protein motif that exhibits binding specificity, refers to the DNA sequence , to which the domain is specific...
which occurs only rarely in a genome. An example is NotI, which cuts after the first GC of a 5'-GCGGCCGC-3' sequence; restriction enzymes with seven and eight base pair recognition sequences are often also called rare-cutter enzymes (six bp recognition sequences are much more common).
For example, rare-cutter enzymes with 7-nucleotide recognition sites cut once every 47 bp (16,384 bp), and those with 8-nucleotide recognition sites cut every 48 bp (65,536 bp) respectively. They are used in top-down mapping
DNA sequencing
DNA sequencing includes several methods and technologies that are used for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA....
to cut a chromosome into chunks of these sizes on average.