Ponceau 2R
Encyclopedia
Ponceau 2R, Xylidine ponceau, Ponceau G, Red R, Acid Red 26, Food Red 5, or C.I. 16150 is a red azo dye
Azo compound
Azo compounds are compounds bearing the functional group R-N=N-R', in which R and R' can be either aryl or alkyl. IUPAC defines azo compounds as: "Derivatives of diazene , HN=NH, wherein both hydrogens are substituted by hydrocarbyl groups, e.g. PhN=NPh azobenzene or diphenyldiazene." The more...

 used in histology
Histology
Histology is the study of the microscopic anatomy of cells and tissues of plants and animals. It is performed by examining cells and tissues commonly by sectioning and staining; followed by examination under a light microscope or electron microscope...

 for staining
Staining (biology)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes...

. It is easily soluble in water and slightly in ethanol
Ethanol
Ethanol, also called ethyl alcohol, pure alcohol, grain alcohol, or drinking alcohol, is a volatile, flammable, colorless liquid. It is a psychoactive drug and one of the oldest recreational drugs. Best known as the type of alcohol found in alcoholic beverages, it is also used in thermometers, as a...

. It usually comes as a disodium salt.

Xylidine ponceau is mostly used only in the Masson's trichrome
Masson's trichrome
Masson's trichrome is a three-colour staining protocol used in histology. The recipes evolved from Claude L. Pierre Masson's original formulation to different specific applications, but all are suited for distinguishing cells from surrounding connective tissue....

 stain as a red counterstain
Counterstain
A counterstain is a stain with color contrasting to the principal stain, making the stained structure more easily visible.An example is the malachite green counterstain to the fuchsine stain in the Gimenez staining technique....

, where it imparts orange hue to the red-stained cytoplasm
Cytoplasm
The cytoplasm is a small gel-like substance residing between the cell membrane holding all the cell's internal sub-structures , except for the nucleus. All the contents of the cells of prokaryote organisms are contained within the cytoplasm...

ic structures.

Its other names are:
Acetacid red J,
Acidal ponceau G,
Acid leather red KPR,
Acid leather red P2R,
Acid leather scarlet IRW,
Acid ponceau R,
Acid ponceau 2RL,
Acid ponceau special,
Acid scarlet,
Acid scarlet 2RL,
Acid scarlet 2RN,
Acilan ponceau RRL,
Ahcocid fast scarlet R,
Aizen ponceau RH,
Amacid lake scarlet 2R,
Amacid scarlet 2R,
Brilliant ponceau G,
Calcocid 2RIL,
Calcocid scarlet 2R,
Calcocid scarlet 2RIL,
Calcolake scarlet 2R,
Certicol ponceau MXS,
C.I. Acid red 26,
C.I. Food red 5,
Colacid ponceau special,
Comacid scarlet 2R
D and C red No. 5,
Edicol supra ponceau R,
Fenazo scarlet 2R,
Hexacol ponceau MX,
Hexacol ponceau 2R,
Hidacid scarlet 2R,
Hispacid ponceau R,
Java ponceau 2R,
Kiton ponceau R,
Kiton scarlet 2RC,
Lake ponceau,
Lake scarlet R,
Lake scarlet 2RBN,
Naphthalene lake scarlet R,
Naphthalene scarlet R,
Naphthazine scarlet 2R,
Neklacid red RR,
New ponceau 4R,
Paper red HRR,
Pigment ponceau R,
Ponceau BNA,
Ponceau de xylidine,
Ponceau R,
Ponceau red,
Ponceau RG,
Ponceau xylidine,
1695 Red,
Red for lakes J,
Red R,
Tertracid ponceau 2R,
Xylidine ponceau 3RS,
Xylidine red.
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