NRON also known as ncRNA repressor of the nuclear factor of activated T cells is a non-coding RNA

Non-coding RNA

A non-coding RNA is a functional RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA , non-messenger RNA and functional RNA . The term small RNA is often used for short bacterial ncRNAs...

 involved in repressing NFAT

NFAT

Nuclear factor of activated T-cells is a general name applied to a family of transcription factors shown to be important in immune response. One or more members of the NFAT family is expressed in most cells of the immune system...

. The function of this ncRNA was identified by a large-scale screen of 512 non-coding RNAs discovered in earlier EST

Expressed sequence tag

An expressed sequence tag or EST is a short sub-sequence of a cDNA sequence. They may be used to identify gene transcripts, and are instrumental in gene discovery and gene sequence determination. The identification of ESTs has proceeded rapidly, with approximately 65.9 million ESTs now available in...

 sequencing projects.

Each of the RNAs that were conserved between human and mouse were knocked down

Knocked down

Knocked down may refer to:* A car kit, see CKD.* Being knocked down in combat sports....

 using shRNAs. The resulting cell-lines were screened for changes in activity of NFAT.