Mostafa Ronaghi
Encyclopedia
DNA sequencing
DNA sequencing includes several methods and technologies that are used for determining the order of the nucleotide bases—adenine, guanine, cytosine, and thymine—in a molecule of DNA....
methodology . He earned his Ph.D. from the Royal Institute of Technology
Royal Institute of Technology
The Royal Institute of Technology is a university in Stockholm, Sweden. KTH was founded in 1827 as Sweden's first polytechnic and is one of Scandinavia's largest institutions of higher education in technology. KTH accounts for one-third of Sweden’s technical research and engineering education...
in Sweden in 1998.
He is currently the Chief Technology Officer and Senior Vice President at Illumina
Illumina (company)
Illumina, Inc. is a company incorporated in April 1998 that develops, manufactures and markets integrated systems for the analysis of genetic variation and biological function. Using its technologies, the company provides a line of products and services that serve the sequencing, genotyping and...
. Prior to this position, he was a principal investigator and Senior Research Associate at the Stanford Genome Technology Center at Stanford University
Stanford University
The Leland Stanford Junior University, commonly referred to as Stanford University or Stanford, is a private research university on an campus located near Palo Alto, California. It is situated in the northwestern Santa Clara Valley on the San Francisco Peninsula, approximately northwest of San...
, focusing on developing analytical techniques for molecular diagnostics.
He is principal investigator for several grants including grants from the National Human Genome Research Institute
National Human Genome Research Institute
The National Human Genome Research Institute is a division of the National Institutes of Health, located in Bethesda, Maryland.NHGRI began as the National Center for Human Genome Research , which was established in 1989 to carry out the role of the NIH in the International Human Genome Project...
(NHGRI), part of the National Institutes of Health
National Institutes of Health
The National Institutes of Health are an agency of the United States Department of Health and Human Services and are the primary agency of the United States government responsible for biomedical and health-related research. Its science and engineering counterpart is the National Science Foundation...
for the development of array-based Pyrosequencing
Pyrosequencing
Pyrosequencing is a method of DNA sequencing based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides...
.
He is an inventor of Pyrosequencing
Pyrosequencing
Pyrosequencing is a method of DNA sequencing based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides...
technology and co-founded Pyrosequencing AB in 1997 (renamed to Biotage in 2003), and co-invented Molecular Inversion Probe assay and co-founded ParAllele BioScience to develop this multiplexed technology for genetic testing. ParAllele was acquired by Affymetrix
Affymetrix
Affymetrix is a company that manufactures DNA microarrays; it is based in Santa Clara, California, United States. The company was founded by Dr. Stephen Fodor in 1992. It began as a unit in Affymax N.V...
in May 2005.
In 2005, he co-founded Nextbio
Nextbio
NextBio is a privately owned software company that provides a platform for drug companies and life science researchers to search, discover, and share knowledge across public and proprietary data...
, a search engine for life science data. He also serves on the board of directors of Microchip Biotechnologies and Aurora Biofuels. In 2008 he co-founded Avantome, a DNA sequencing company acquired by Illumina July 2008.
Publications
Among his approximately 50 peer-reviewed journal articles are the publications describing Pyrosequencing and Molecular Inversion Probe assays and other techniques:- Absalan F, Ronaghi M. "Molecular inversion probe assay." Methods in Molecular BiologyMethods in Molecular BiologyMethods in Molecular Biology is a book series from publisher Humana Press that presents biomedical and life science research methods and protocols. The book series was introduced by series editor Dr. in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. ...
2007;396:315-30. PMID 18025701 - Hardenbol P, Banér J, Jain M, Nilsson M, Namsaraev EA, Karlin-Neumann GA, Fakhrai-Rad H, Ronaghi M, Willis TD, Landegren U, Davis RW. "Multiplexed genotyping with sequence-tagged molecular inversion probes." Nature BiotechnologyNature BiotechnologyNature Biotechnology is an academic journal covering the science and business of biotechnology.Nature Biotechnology is a continuation of Bio/Technology , which was founded in 1983 and renamed in 1996. It is published monthly by the Nature Publishing Group, a division of Macmillan Publishers Ltd...
2003 Jun;21(6):673-8. Epub 2003 May 5. PMID 12730666 - "Prediction of protein orientation upon immobilization on biological and nonbiological surfaces." Proceedings of the National Academy of SciencesProceedings of the National Academy of SciencesThe Proceedings of the National Academy of Sciences of the United States of America, usually referred to as PNAS, is the official journal of the United States National Academy of Sciences...
2006 Oct 3;103(40):14773-8. Epub 2006 Sep 25. PMID 17001006 - Ronaghi M. "Pyrosequencing sheds light on DNA sequencing." Genome ResearchGenome ResearchGenome Research is a peer-reviewed scientific journal published by Cold Spring Harbor Laboratory Press. The focus of the journal is on genome-wide studies in any organism, including single gene studies that are placed in a genomic context. This scope includes both experimental and computational...
2001 Jan;11(1):3-11. PMID 11156611. - Ronaghi M, Uhlén M, Nyrén P. "A sequencing method based on real-time pyrophosphate." ScienceScienceScience is a systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions about the universe...
1998 Jul 17;281(5375):363, 365. PMID 9705713. - Ronaghi M, Karamohamed S, Pettersson B, Uhlén M, Nyrén P. "Real-time DNA sequencing using detection of pyrophosphate release." Analytical Biochemistry 1996 Nov 1;242(1):84-9. PMID 8923969.
He also holds about 20 patents.