Middlebrook 7H10 Agar
Encyclopedia
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis
. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks.
Mycobacterium tuberculosis
Mycobacterium tuberculosis is a pathogenic bacterial species in the genus Mycobacterium and the causative agent of most cases of tuberculosis . First discovered in 1882 by Robert Koch, M...
. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Composition
- Ammonium SulfateAmmonium sulfateAmmonium sulfate , 2SO4, is an inorganic salt with a number of commercial uses. The most common use is as a soil fertilizer. It contains 21% nitrogen as ammonium cations, and 24% sulfur as sulfate anions...
- Monopotassium phosphateMonopotassium phosphateMonopotassium phosphate -- 24 -- is a soluble salt which is used as a fertilizer, a food additive and a fungicide. It is a source of phosphorus and potassium. It is also a buffering agent...
- Disodium phosphateDisodium phosphateDisodium hydrogen phosphate is a sodium salt of phosphoric acid. It is a white powder that is highly hygroscopic and water soluble. It is therefore used commercially as an anti-caking additive in powdered products. It is also known as disodium hydrogen orthophosphate, sodium hydrogen phosphate...
- Sodium Citrate
- Magnesium SulfateMagnesium sulfateMagnesium sulfate is a chemical compound containing magnesium, sulfur and oxygen, with the formula MgSO4. It is often encountered as the heptahydrate epsomite , commonly called Epsom salt, from the town of Epsom in Surrey, England, where the salt was distilled from the springs that arise where the...
- Calcium ChlorideCalcium chlorideCalcium chloride, CaCl2, is a salt of calcium and chlorine. It behaves as a typical ionic halide, and is solid at room temperature. Common applications include brine for refrigeration plants, ice and dust control on roads, and desiccation...
- Zinc Sulfate
- Copper Sulfate
- L-Glutamic Acid (sodium salt)
- Ferric Ammonium Citrate
- Pyridoxine Hydrochloride
- Biotin
- Malachite Green
- Agar
Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks.