MARCM
Encyclopedia
Mosaic analysis with a repressible cell marker, or MARCM, is a genetics
technique for creating individually labeled homozygous cells in an otherwise heterozygous Drosophila melanogaster
. This technique relies on recombination during mitosis mediated by FLP-FRT Recombination
. As one copy of a gene, provided by the balancer chromosome
, is often enough to rescue a mutant phenotype
, MARCM clones can be used to study a mutant phenotype in an otherwise normal animal.
Genetics
Genetics , a discipline of biology, is the science of genes, heredity, and variation in living organisms....
technique for creating individually labeled homozygous cells in an otherwise heterozygous Drosophila melanogaster
Drosophila melanogaster
Drosophila melanogaster is a species of Diptera, or the order of flies, in the family Drosophilidae. The species is known generally as the common fruit fly or vinegar fly. Starting from Charles W...
. This technique relies on recombination during mitosis mediated by FLP-FRT Recombination
FLP-FRT Recombination
In genetics, FLP-FRT recombination is a site-directed recombination technology used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-Lox recombination...
. As one copy of a gene, provided by the balancer chromosome
Balancer chromosome
Balancer Chromosomes are special, modified chromosomes used for genetically screening a population of organisms to select for heterozygotes. A balancer chromosomes can be used as a genetic tool used to prevent crossing over between homologous chromosomes during meiosis...
, is often enough to rescue a mutant phenotype
Phenotype
A phenotype is an organism's observable characteristics or traits: such as its morphology, development, biochemical or physiological properties, behavior, and products of behavior...
, MARCM clones can be used to study a mutant phenotype in an otherwise normal animal.