Light Green SF yellowish
Encyclopedia
Light Green SF yellowish, or Light Green, Acid Green, Lissamine green SF, Acid Green 5, Food Green 2, FD&C Green no. 2, Green No. 205, Acid Brilliant Green 5, Pencil Green SF, or C.I. 42095, is a green triarylmethane dye
. It is used in histology
for staining
collagen
; for that purpose it is a standard dye in North America. In Masson's trichrome
it is used as a counterstain
to acid fuchsin. It is a critical component of Papanicolaou stain
s together with eosin Y
and bismarck brown Y
. It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm.
The dye is not very durable — it has a tendency to fade. When fading is to be avoided, it is replaced with Fast Green FCF
, which also has more brilliant color. Fast Green FCF can also substitute Light Green SF yellowish in many other procedures.
Lissamine green dye can be used to check the health of the anterior surfaces of the eye. It is available on a steret. The steret is wet with saline and then the dye is dropped into the lower fornix. The dye shows up conjunctival staining similar to rose bengal dye but it does not sting like rose bengal does.
Triarylmethane dye
Triarylmethane dyes are synthetic organic compounds containing triphenylmethane backbones. As dyes, these compounds are intensely colored. Many of these dyes undergo reactions in response to acid and base, and thus serve as pH indicators....
. It is used in histology
Histology
Histology is the study of the microscopic anatomy of cells and tissues of plants and animals. It is performed by examining cells and tissues commonly by sectioning and staining; followed by examination under a light microscope or electron microscope...
for staining
Staining (biology)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes...
collagen
Collagen
Collagen is a group of naturally occurring proteins found in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content...
; for that purpose it is a standard dye in North America. In Masson's trichrome
Masson's trichrome
Masson's trichrome is a three-colour staining protocol used in histology. The recipes evolved from Claude L. Pierre Masson's original formulation to different specific applications, but all are suited for distinguishing cells from surrounding connective tissue....
it is used as a counterstain
Counterstain
A counterstain is a stain with color contrasting to the principal stain, making the stained structure more easily visible.An example is the malachite green counterstain to the fuchsine stain in the Gimenez staining technique....
to acid fuchsin. It is a critical component of Papanicolaou stain
Papanicolaou stain
Papanicolaou stain is a multichromatic staining histological technique developed by George Papanikolaou, the father of cytopathology....
s together with eosin Y
Eosin Y
Eosin Y is a form of eosin....
and bismarck brown Y
Bismarck brown Y
Bismarck brown Y is a diazo dye. It is used in histology for staining tissues. It stains acid mucins to yellow color. It can be used with live cells...
. It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm.
The dye is not very durable — it has a tendency to fade. When fading is to be avoided, it is replaced with Fast Green FCF
Fast Green FCF
Fast Green FCF, also called Food green 3, FD&C Green No. 3, Green 1724, Solid Green FCF, and C.I. 42053, is a sea green triarylmethane food dye. Its E number is E143....
, which also has more brilliant color. Fast Green FCF can also substitute Light Green SF yellowish in many other procedures.
Lissamine green dye can be used to check the health of the anterior surfaces of the eye. It is available on a steret. The steret is wet with saline and then the dye is dropped into the lower fornix. The dye shows up conjunctival staining similar to rose bengal dye but it does not sting like rose bengal does.