Janus green
Encyclopedia
Janus Green B is a basic dye
Dye
A dye is a colored substance that has an affinity to the substrate to which it is being applied. The dye is generally applied in an aqueous solution, and requires a mordant to improve the fastness of the dye on the fiber....

 and vital stain
Vital stain
A vital stain is a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties...

 used in histology. It is also used to stain mitochondria
Mitochondrion
In cell biology, a mitochondrion is a membrane-enclosed organelle found in most eukaryotic cells. These organelles range from 0.5 to 1.0 micrometers in diameter...

supravitally.

The indicator Janus Green B changes colour according to the amount of oxygen present.
  • Oxygen present: Indicator oxidised to blue colour
  • Oxygen absent: Indicator reduced to pink colour
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