HPCE-based viral titer assay
Encyclopedia
HPCE is widely used in the analysis of biomolecules. Recently, a further application, to titer baculovirus, has been developed using HPCE technology and a proprietary buffer system belonging to the UK company deltaDOT. The application has been named deltaTITER and uses a multi-pixel detection system to quantify the amount of virus present in a sample. It offers several advantages over other approaches, the most crucial being the tremendous saving in time and resource, especially over the traditional plaque assay. The assay is highly reproducible, with a % RSD of less than 6. The result is presented as an absorption peak, and quantified using bespoke software.