HIS3
Encyclopedia
The HIS3 gene, found in the Saccharomyces cerevisiae
yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase
which catalyses the sixth step in histidine biosynthesis. It is analogous to hisB
in Escherichia coli.
s analogous gene, hisB
allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening
to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place (Joung et al., 2000)
Saccharomyces cerevisiae
Saccharomyces cerevisiae is a species of yeast. It is perhaps the most useful yeast, having been instrumental to baking and brewing since ancient times. It is believed that it was originally isolated from the skin of grapes...
yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase
Imidazoleglycerol-phosphate dehydratase
In enzymology, an imidazoleglycerol-phosphate dehydratase is an enzyme that catalyzes the chemical reactionHence, this enzyme has one substrate, D-erythro-1-glycerol 3-phosphate, and two products, 3--2-oxopropyl phosphate and H2O.This enzyme belongs to the family of lyases, specifically the...
which catalyses the sixth step in histidine biosynthesis. It is analogous to hisB
HisB
The hisB gene, found in the enterobacteria , in Campylobacter jejuni and in Xylella/Xanthomonas encodes a protein involved in catalysis of two step in histidine biosynthesis , namely the bifunctional Imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase.The former function , found at the...
in Escherichia coli.
Exploits
Mutations in Escherichia coliEscherichia coli
Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms . Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls...
s analogous gene, hisB
HisB
The hisB gene, found in the enterobacteria , in Campylobacter jejuni and in Xylella/Xanthomonas encodes a protein involved in catalysis of two step in histidine biosynthesis , namely the bifunctional Imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase.The former function , found at the...
allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening
Two-hybrid screening
Two-hybrid screening is a molecular biology technique used to discover protein–protein interactions and protein–DNA interactions by testing for physical interactions between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of...
to allow the survival of E. coli in which a desired protein-DNA or protein-protein interaction is taking place (Joung et al., 2000)