Filter fluorometer
Encyclopedia
A filter fluorometer is a type of fluorometer
that may be employed in fluorescence spectroscopy
.
In the fluorometer, a light source emits light of an excitation wavelength that is relevant to the compound to be measured. Filter fluorometers produce specific excitation and emission wavelengths by using optical filters. The filter blocks other wavelengths but transmits wavelengths relevant to the compound. The light passes through the sample to be measured, and a certain wavelength is absorbed while a longer wavelength is emitted. The emitted light is measured by a detector. By changing the optical filter, different substances can be measured.
and the excitation spectrum. The primary filter is selected to transmit only the wavelengths from the emission spectrum and excitation spectrum that overlap.
A new type of filter fluorometer. "This is a very sensitive instrument. . . the analytical time needed to measure sample fluorescence is very short." It is compact and light weight, yet retains the sensitivity of a laboratory system.
The case houses user changeable source and detector filter sets, making it suitable for a wide range of applications.
It also differs from previous models because it includes additional memory storage, convenient SD card memory modules, USB port, colour graphical display, and a longer lasting NiMH battery.
The portable nature of this type of fluorometer makes it suitable for field use especially for ground water mapping, pollution monitoring, mixing zone studies, time of travel/flow monitoring, contaminant tracking and the studying of algal blooms.
This type of fluorometer is made by Opti-Sciences, Inc. Hudson, NH USA
There are a number of filter fluorometer types. They range from Plant Science based, which are used extensively to detect, measure and analyse fluorescence from chlorophyll during the photosynthesis process, to general purpose fluorometers tuned to the specific fluorescence porperties of target samples. Most common targets are Chlorophyll, Fluorescein, Rhodamine, Green Fluorescent Protein, and Colorated Dissolved Organic Matter (CDOM). Customized dyes and taggants have been developed for a wide variety of applications including; waste water tracking, biologic activity, part inspection, subsea pipeline leak detection, counterfeit detection, genetic tracing, disease and health studies, and DNA replication.
Additional types include Opti-Sciences' GFP-Meter, GFL-1, Seapoint SCF, SRF,SFF and SUVF, and a variety of OEM designs.
Turner 110 is manufactured by Turner Designs
GFP & GFL-1 are manufactured by Opti-Sciences, Inc.
Seapoint SCF, SRF, SFF, SUVF, are manufactured by Seapoint Sensors Inc.
In addition to the filter fluorometers shown above, fluorometers are used extensively in Life Science research for various assays such as Alkaline Phosphatase, Bacterial Viability, B-Galactosidase, Cell Proliferation, RNA Quantitation, DNA Quantitation, Enzyme Assays, Protein Quantitation, and Reporter Gene Assays.
QuantiFluor is manufactured by Promega Corporation
Fluorometer
A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. These parameters are used to identify the presence and the amount of specific molecules in a medium...
that may be employed in fluorescence spectroscopy
Fluorescence spectroscopy
Fluorescence spectroscopy aka fluorometry or spectrofluorometry, is a type of electromagnetic spectroscopy which analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit...
.
In the fluorometer, a light source emits light of an excitation wavelength that is relevant to the compound to be measured. Filter fluorometers produce specific excitation and emission wavelengths by using optical filters. The filter blocks other wavelengths but transmits wavelengths relevant to the compound. The light passes through the sample to be measured, and a certain wavelength is absorbed while a longer wavelength is emitted. The emitted light is measured by a detector. By changing the optical filter, different substances can be measured.
Applications
Filter fluorometers can be highly sensitive, so are well suited to precise scientific research. The optical filters are relatively inexpensive and are easy to change, so filter fluorometers are commonly used in experimental applications which repeatedly measure different compounds.Filters
There are two filters for the fluorometer:- The primary filter or excitation filter or incident light filter isolates the wavelength that will cause the compound to fluoresceFluorescenceFluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength. It is a form of luminescence. In most cases, emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation...
(the incident light). - The secondary filter isolates the desired emitted light (fluorescent light).
Choosing the correct filters
The proper selection of filters requires familiarity with the emission spectrumEmission spectrum
The emission spectrum of a chemical element or chemical compound is the spectrum of frequencies of electromagnetic radiation emitted by the element's atoms or the compound's molecules when they are returned to a lower energy state....
and the excitation spectrum. The primary filter is selected to transmit only the wavelengths from the emission spectrum and excitation spectrum that overlap.
Types
A new type of filter fluorometer. "This is a very sensitive instrument. . . the analytical time needed to measure sample fluorescence is very short." It is compact and light weight, yet retains the sensitivity of a laboratory system.
The case houses user changeable source and detector filter sets, making it suitable for a wide range of applications.
It also differs from previous models because it includes additional memory storage, convenient SD card memory modules, USB port, colour graphical display, and a longer lasting NiMH battery.
The portable nature of this type of fluorometer makes it suitable for field use especially for ground water mapping, pollution monitoring, mixing zone studies, time of travel/flow monitoring, contaminant tracking and the studying of algal blooms.
This type of fluorometer is made by Opti-Sciences, Inc. Hudson, NH USA
- Turner 110
There are a number of filter fluorometer types. They range from Plant Science based, which are used extensively to detect, measure and analyse fluorescence from chlorophyll during the photosynthesis process, to general purpose fluorometers tuned to the specific fluorescence porperties of target samples. Most common targets are Chlorophyll, Fluorescein, Rhodamine, Green Fluorescent Protein, and Colorated Dissolved Organic Matter (CDOM). Customized dyes and taggants have been developed for a wide variety of applications including; waste water tracking, biologic activity, part inspection, subsea pipeline leak detection, counterfeit detection, genetic tracing, disease and health studies, and DNA replication.
Additional types include Opti-Sciences' GFP-Meter, GFL-1, Seapoint SCF, SRF,SFF and SUVF, and a variety of OEM designs.
Turner 110 is manufactured by Turner Designs
GFP & GFL-1 are manufactured by Opti-Sciences, Inc.
Seapoint SCF, SRF, SFF, SUVF, are manufactured by Seapoint Sensors Inc.
- [Promega]http://www.fluorometer.com QuantiFluor-P Fluorometer
In addition to the filter fluorometers shown above, fluorometers are used extensively in Life Science research for various assays such as Alkaline Phosphatase, Bacterial Viability, B-Galactosidase, Cell Proliferation, RNA Quantitation, DNA Quantitation, Enzyme Assays, Protein Quantitation, and Reporter Gene Assays.
QuantiFluor is manufactured by Promega Corporation