Discovery

Eos was isolated from the coral Lobophyllia hemprichii in a large-scale screen for PAFPs.

Properties

Eos undergoes an irreversible photoconversion in response to 390 nm light. Prior to this conversion, it exhibits a green emission peak at 516 nm. Following conversion, it has a red emission peak at 581 nm.

Wild-type Eos is a tetrameric protein. It has been modified to generate a monomeric form (referred to as mEos) that is more amenable for experiments involving labeling of a single molecule.

Properties
Photoconversion wavelength	390 nm
Green absorbance peak	506 nm
Green emission peak	516 nm
Red absorbance peak	571 nm
Red emission peak	581 nm
Green brightness*	1.3X
Red brightness*	0.7X
*Brightness values are relative to EGFP.

Applications

Like other PAFPs, Eos can be used to optically label single cells for subsequent analysis. Because Eos is available in monomeric forms, it can also be used to observe the kinetics and trafficking of single molecules as a fusion protein

Fusion protein

Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes which originally coded for separate proteins. Translation of this fusion gene results in a single polypeptide with functional properties derived from each of the original proteins...

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