Dilution cloning
Encyclopedia
Dilution cloning describes the process of obtaining a monoclonal
cell population starting from a polyclonal mass of cells.
This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. The number of dilutions will depend on cell number in the starting population, as well as the characteristics of the cell type in question.
After the final dilution has been produced, small batches of the suspension is placed in wells and permitted to grow, ideally forming a monoclonal cell colony.
Monoclonal
Monoclonal cells are defined as a group of cells produced from a single ancestral cell by repeated cellular replication. Thus they can be said to form a single "clone". The process of replication can occur in vivo, or may be stimulated in vitro for laboratory manipulations...
cell population starting from a polyclonal mass of cells.
This is achieved by setting up a series of increasing dilutions of the parent (polyclonal) cell culture. The number of dilutions will depend on cell number in the starting population, as well as the characteristics of the cell type in question.
After the final dilution has been produced, small batches of the suspension is placed in wells and permitted to grow, ideally forming a monoclonal cell colony.