Calycosin
Encyclopedia
Calycosin is a O-methylated isoflavone. It can be isolated from Astragalus membranaceus Bge. var. mongholicus and Trifolium pratense (red clover).

Biosynthesis

Isoflavone 3'-hydroxylase
Isoflavone 3'-hydroxylase
In enzymology, an isoflavone 3'-hydroxylase is an enzyme that catalyzes the chemical reactionThe 4 substrates of this enzyme are formononetin, NADPH, H+, and O2, whereas its 3 products are calycosin, NADP+, and H2O....

 uses formononetin
Formononetin
Formononetin is an O-methylated isoflavone. It is found in a number of plants and herbs like the red clover. Along with other phytoestrogens, it predominantly occurs in leguminous plants and Fabaceae, particularly in beans, such as green beans, lima beans, soy and many others, as the free aglycone...

, NADPH, H+ and O2 to produce calycosin, NADP+ and H2O.
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