Buffered charcoal yeast extract agar
Encyclopedia
Buffered charcoal yeast extract (BCYE) agar
is a selective growth medium used to culture
or grow certain bacteria
, particularly the Gram-negative
species Legionella pneumophila
.
It has also been used for the recovery of Acanthamoeba keratitis
.
Agar
Agar or agar-agar is a gelatinous substance derived from a polysaccharide that accumulates in the cell walls of agarophyte red algae. Throughout history into modern times, agar has been chiefly used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture medium...
is a selective growth medium used to culture
Microbiological culture
A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions. Microbial cultures are used to determine the type of organism, its abundance in the sample being tested,...
or grow certain bacteria
Bacteria
Bacteria are a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria have a wide range of shapes, ranging from spheres to rods and spirals...
, particularly the Gram-negative
Gram-negative
Gram-negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol. In a Gram stain test, a counterstain is added after the crystal violet, coloring all Gram-negative bacteria with a red or pink color...
species Legionella pneumophila
Legionella pneumophila
Legionella pneumophila is a thin, ærobic, pleomorphic, flagellated, non-spore forming, Gram-negative bacterium of the genus Legionella. L. pneumophila is the primary human pathogenic bacterium in this group and is the causative agent of legionellosis or Legionnaires' disease.-Characterization:L...
.
It has also been used for the recovery of Acanthamoeba keratitis
Acanthamoeba keratitis
Acanthamoeba keratitis is a rare disease in which amoebae invade the cornea of the eye.-Causes:In the United States, it is nearly always associated with contact lens use, as Acanthamoeba can survive in the space between the lens and the eye...
.