Bordet-Gengou agar
Encyclopedia
Bordet-Gengou agar is a type of agar plate
optimized to isolate Bordetella
, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or penicillin and sometimes nicotinamide. The potato extract provided nitrogen and vitamins, and potato starch absorbed fatty acids present in nasal secretions or collection-swab cotton that inhibited growth; glycerol was a carbon source. Medical Microbiology, 4th edition states that Regan-Lowe medium (containing charcoal, blood, and antibiotic) has replaced Bordet-Gengou medium as the medium of choice for routine Bordetella pertussis
incubation.
Bordetella
bacteria were difficult to culture; Jules Bordet
and Octave Gengou
invented the first version in order to isolate the bacillus, named Bordet-Gengou in 1906, that they believed was associated with whooping cough. In 1909 it was not yet established that the bacillus was solely the cause.
Agar plate
An agar plate is a Petri dish that contains a growth medium used to culture microorganisms or small plants like the moss Physcomitrella patens.Selective growth compounds may also be added to the media, such as antibiotics....
optimized to isolate Bordetella
Bordetella
Bordetella is a genus of small , Gram-negative coccobacilli of the phylum proteobacteria. Bordetella species, with the exception of B. petrii, are obligate aerobes as well as highly fastidious, or difficult to culture. Three species are human pathogens ; one of these Bordetella is a genus of small...
, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or penicillin and sometimes nicotinamide. The potato extract provided nitrogen and vitamins, and potato starch absorbed fatty acids present in nasal secretions or collection-swab cotton that inhibited growth; glycerol was a carbon source. Medical Microbiology, 4th edition states that Regan-Lowe medium (containing charcoal, blood, and antibiotic) has replaced Bordet-Gengou medium as the medium of choice for routine Bordetella pertussis
Bordetella pertussis
Bordetella pertussis is a Gram-negative, aerobic coccobacillus of the genus Bordetella, and the causative agent of pertussis or whooping cough. Unlike B. bronchiseptica, B. pertussis is non-motile. Its virulence factors include pertussis toxin, filamentous hæmagglutinin, pertactin, fimbria, and...
incubation.
Bordetella
Bordetella
Bordetella is a genus of small , Gram-negative coccobacilli of the phylum proteobacteria. Bordetella species, with the exception of B. petrii, are obligate aerobes as well as highly fastidious, or difficult to culture. Three species are human pathogens ; one of these Bordetella is a genus of small...
bacteria were difficult to culture; Jules Bordet
Jules Bordet
Jules Jean Baptiste Vincent Bordet was a Belgian immunologist and microbiologist. The bacterial genus Bordetella is named after him.-Biography:Bordet was born at Soignies, Belgium...
and Octave Gengou
Octave Gengou
Octave Gengou was a Belgian bacteriologist. He researched with Jules Bordet the Bordetella pertussis bacteria.- Biography :...
invented the first version in order to isolate the bacillus, named Bordet-Gengou in 1906, that they believed was associated with whooping cough. In 1909 it was not yet established that the bacillus was solely the cause.