Aniline Blue WS
Encyclopedia
Aniline Blue WS, also called aniline blue, China blue, or Soluble blue, is a mixture of methyl blue
Methyl blue
Methyl blue, also known as Cotton blue, Helvetia blue, Acid blue 93, or C.I. 42780, is a chemical compound with the molecular formula C37H27N3Na2O9S3. It is used as a stain in histology. Methyl blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol...

 and water blue
Water blue
Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections...

. It may also be either one of them.

Aniline blue or its constituents are used to stain
Staining (biology)
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes...

 collagen
Collagen
Collagen is a group of naturally occurring proteins found in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content...

, as the fibre stain in Masson's trichrome
Masson's trichrome
Masson's trichrome is a three-colour staining protocol used in histology. The recipes evolved from Claude L. Pierre Masson's original formulation to different specific applications, but all are suited for distinguishing cells from surrounding connective tissue....

.

It can be used in the Mallory's connective tissue
Connective tissue
"Connective tissue" is a fibrous tissue. It is one of the four traditional classes of tissues . Connective Tissue is found throughout the body.In fact the whole framework of the skeleton and the different specialized connective tissues from the crown of the head to the toes determine the form of...

 stain and Gömöri trichrome stain
Gomori trichrome stain
Gömöri trichrome stain is a stain used on muscle tissue.It can be used to test for certain forms of mitochondrial myopathy.It is named for George Gömöri, who developed it in 1950.-External links:...

. It is used in differential staining
Differential staining
Differential Staining is a general term that can refer to a number of specific processes. Generally, it is used to describe staining processes which use more than one chemical stain...

.
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