Perls' Prussian blue
Encyclopedia
Prussian blue is a common stain used by pathologists to detect the presence of iron in biopsy specimens, such as deposits of storage ferritin
in bone marrow biopsy samples.
The original stain formula, known historically (1867) as "Perls' Prussian blue" after its inventor, German pathologist Max Perls (1843-1881), used separate solutions of potassium ferrocyanide and acid to stain tissue (these are now used combined, just before staining). Iron deposits in tissue then form the insoluble Prussian blue
dye (a complex hydrated ferric ferrocyanide substance) in place, and are visualized the microscopically as blue or purple deposits within cells. The formula is also known as Perls Prussian blue and (incorrectly) as Perl's Prussian blue.
Ferritin
Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The amount of ferritin stored reflects the amount of iron stored. The protein is produced by almost all living organisms, including bacteria, algae and higher plants, and animals...
in bone marrow biopsy samples.
The original stain formula, known historically (1867) as "Perls' Prussian blue" after its inventor, German pathologist Max Perls (1843-1881), used separate solutions of potassium ferrocyanide and acid to stain tissue (these are now used combined, just before staining). Iron deposits in tissue then form the insoluble Prussian blue
Prussian blue
Prussian blue is a dark blue pigment with the idealized formula Fe718. Another name for the color Prussian blue is Berlin blue or, in painting, Parisian blue. Turnbull's blue is the same substance but is made from different reagents....
dye (a complex hydrated ferric ferrocyanide substance) in place, and are visualized the microscopically as blue or purple deposits within cells. The formula is also known as Perls Prussian blue and (incorrectly) as Perl's Prussian blue.