Folin's reagent
Encyclopedia
Folin's reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used to measure levels of amine
s and amino acid
s. The reagent produces a bright red color in alkaline solutions and is also fluorescent
.
This should not be confused with Folin-Ciocalteu reagent
, which is a mixture of sodium tungstate
and sodium molybdate
that is used to detect phenolic
compounds.
Amine
Amines are organic compounds and functional groups that contain a basic nitrogen atom with a lone pair. Amines are derivatives of ammonia, wherein one or more hydrogen atoms have been replaced by a substituent such as an alkyl or aryl group. Important amines include amino acids, biogenic amines,...
s and amino acid
Amino acid
Amino acids are molecules containing an amine group, a carboxylic acid group and a side-chain that varies between different amino acids. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen...
s. The reagent produces a bright red color in alkaline solutions and is also fluorescent
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength. It is a form of luminescence. In most cases, emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation...
.
This should not be confused with Folin-Ciocalteu reagent
Folin-Ciocalteu reagent
The Folin–Ciocalteu reagent or Folin's phenol reagent or Folin–Denis reagent, also called the Gallic Acid Equivalence method , is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric assay of phenolic and polyphenolic antioxidants...
, which is a mixture of sodium tungstate
Sodium tungstate
Sodium tungstate, Na2WO4, a tungstate of sodium, is useful as a source of tungsten. It is prepared from tungsten ores used to manufacture tungsten by reducing it....
and sodium molybdate
Sodium molybdate
Sodium molybdate, Na2MoO4, is useful as a source of molybdenum. It is often found as the dihydrate, Na2MoO4·2H2O.The molybdate anion is tetrahedral. Two sodium cations coordinate with every one anion.-History:...
that is used to detect phenolic
Phenolic
Phenolic is an adjective and a substantive that may apply to :* Natural phenolics and polyphenols, two related classes of natural compounds found in plants* Phenol , a colorless crystalline solid and aromatic compound...
compounds.