Hi everyone, here's my situation:

I run tests on stomatal responses and aquire over 500 images per day in a single folder, and I need to create sequential difference images.

Basically, I need to subract image 1 from image 2 then image 2 from image 3 and image 3 from image 4 and so on for all 500 images, add false color, then save them all to a new folder.

Is there a plugin I could use, or a macro? I'm new to ImageJ and don't know where to begin.

Thanks for the help, Lauren