Centrifuge
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sciencequip
Hi
I was seaprating the cells from the medium by centrifuging at 4,000rpm. but i think, from the supernatant, it does not look like the cells are seaprated well from the broth. so i centrifuged the supernatant again,then I got some pellets from centrifugation of supernatant. why does it happen ? is that speed enough for separating the pellet from supernatant for one time doing.
or is it because of the culture medium I used ? I used large granules of peptone for growing yeasts, is it not suitable for yeasts ?
please share your thoughts, much appreciated!
thank you
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