Hepatocyte
Hepatocyte attachment
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Goldenbogen

Goldenbogen
7/15/09

Hello,

I started working with primary rat hepatocytes. I managed to isolate them (more then 80 % vitality) and put them in culture. However, cells attach quite slow to the plates:

I use Greiner 96 well plates, Collagen1 coated.
I want to change the medium (William's medium, 10%FBS, Pen/Strep, Glutamine, 10 ug/ml Insulin) after 4 h of seeding. But cells havn"t attached properly after this time period.

Furthermore, cells are kept on ice for at least one hour before seeding because our in vivo lab is not at the same place as our in vitro one.

I'd be happy to get some helpful advises for a better cell attachment.

Cheers

Jan
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